Your Reagent Warehouse for COVID-19 Vaccine Development

Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide. The SARS-CoV-2 pandemic presents an extraordinary challenge to the lives and health of the global population, resulting in a total of over 200 million diagnosed cases and 4.9 million death (as of October 2021). It also significantly affected society, economics and the environment around the world and changed everyone's life.

As the virus spreads globally, the continuous emergence of new mutant strains escalated the challenge on humans. To better track and inform the viral variants, the World Health Organization (WHO) characterized several variants as "Variants of Concern (VOCs) " and "Variants of Interest (VOIs) " based on the risk posed to global public health. Today, of hundreds of variants that evolved from the original wild-type virus, Delta (PANGO lineage B.1.617.2/AY) are dominantly circulating and shaping the trend, reported to be responsible for most of the cases worldwide.

Vaccination is the cornerstone to prevent and control the spread of infectious diseases. Despite the importance of mask-wearing and social distancing, immunization by vaccines remains the most cost-effective way to protect our health. For this reason, vaccine development and production are at the center of each nation's public health policy.

ACROBiosystems is a life science company that provides reagents for the global vaccine industry. Having mobilized forces to develop SARS-CoV-2 recombinant antigens since the pandemic and accumulated mature technology and experiences with the virus and vaccines, we supply 200+ products and customized services that cover all the dominant strains of SARS-CoV-2 and support vaccine development of all technological platforms.

>>> Download the poster about core research tools for COVID-19 vaccine R&D

During the special time of need, we will continue to track the epidemic and are determined to facilitate the trial and application of the vaccines.

An Overview of Different COVID-19 Vaccines

In more than a year after the virus outbreak, various scientific research units and vaccine companies have worked to successfully develop a variety of COVID-19 vaccines. The vaccines can be classified by different technological platforms into whole virus vaccines, subunit vaccines, viral vector vaccines and gene vaccines (Fig. 1). Currently, 7 vaccines have obtained the WHO's emergency use authorization to be used in massivevaccination on a global scale, and over 300 vaccines are under development in pre-clinical or clinical trial stage.

Platforms of COVID-19 Vaccines

Recombinant Subunit Vaccines

Protein subunit

Virus-like particles (VLP)

Nucleic Acid Vaccines

DNA based

RNA based

Viral Vector Vaccines

Non-replicating viral vector

Replicating viral vector

Whole Virus Vaccines

Inactivated

Live-attenuated

Regulation of COVID-19 Vaccines Development and Licensure

Workflow for Vaccine R&D

1 Production and QC

(1)Antigen Detection:

Whole virus/Protein based:SDS-PAGE, MS, ELISA;

Virus vectored/ Nucleic acid:Western Blot, ELISA, FACS;

(2)Affinity Detection:

Protein based:ELISA, SPR, BLI;

(3)Efficacy evaluation:

IgG titer detection:ELISA Neutralizing Ab titer detection cVNT, pVNT, sVNT.

2 Preclinical Study

(1)Efficacy evaluation

①In vivo efficacy evaluation:

★Humoral immunity:

ⅠMouse/Monkey IgG titer detection

ⅡNeutralizing Ab titer detection

★Cellular immunity:Elispot

(2)In vitro efficacy evaluation:

①Antigen Detection

3 Clinical Trial Phase I&II

4 Clinical Trial Phase III

(1)Efficacy evaluation

①In vivo efficacy evaluation:

★Humoral immunity:

ⅠHuman IgG titer detection

ⅡNeutralizing Ab titer detection

★Cellular immunity:Elispot

②In vitro efficacy evaluation:Antigen Detection

(2)Toxicity evaluation

①ADE/VED detection:IgG isotype titer mesurement

>>> Development and Licensure of Vaccines to Prevent COVID-19

ACROBiosystems Products & Services for COVID-19 Vaccine R&D

Antigen Detection

Antigen Bioactivity Characterization

Immune Persistence Research

Critical reagents for vaccine development

Antigen Detection

• Super stable SARS-CoV-2 spike trimer

• RBD/S trimer antigen quantitative detection kit

The ideal control for antigen detection:Super stable SARS-CoV-2 spike trimer

Closely resemble the natural conformation: Introduction of 6P mutations on S2 stabilizes spike protein in the pre-fusion state.
High trimer purity (>90%) verified by SDS-PAGE, SEC-MALS and Negative Stain Electron Microscopy (NS-EM).
Provide trimer mutants of all VOCs, VOIs and other dominant strains.
Suitable as control in antigen detection tests.

Product List

Lineage	Cat.No.	Mutation	Tag	Preorder/Order
SARS-CoV-2 Wild Type	SPN-C52H9	NA	His Tag	Order
	SPN-C82E9	NA	His & Avitag™	Order
SARS-CoV-2    OmicronNEW B.1.1.529	SPN-C82Ee	A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F	His Tag & Avi Tag	Order
	SPN-C52Hz	A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F	His Tag	Order
SARS-CoV-2 Delta | B.1.617.2	SPN-C52He	T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N	His Tag	Order
	SPN-CR2H7	T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N	His Tag	Order
	SPN-C82Ec	T19R, G142D, EF156-157del, R158G, L452R, T478K, D614G, P681R, D950N	His & Avitag™	Order
SARS-CoV-2 Delta Plus | AY.1 | AY.2 | AY.3	SPN-C52Ht	T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N	His Tag	Order
	SPN-C82Ei	T19R, V70F, FR157-158Del, A222V, W258L, K417N, L452R, T478K, D614G, P681R, D950N	His & Avitag™	Order
SARS-CoV-2 Lambda | C.37	SPN-C52Hs	G75V, T76I, SYLTPGD 247-253 del, L452Q, F490S, D614G, T859N	His Tag	Order
	SPN-C82Eh	G75V, T76I, SYLTPGD 247-253 del, L452Q, F490S, D614G, T859N	His & Avitag™	Order
SARS-CoV-2 C.1.2	SPN-C52Hb	C136F, Y144del, R190S, D215G, LA242-243del, Y449H, E484K, N501Y, D614G, H655Y, N679K, T716I, T859N	His Tag	Order
	SPN-C82Eq	C136F, Y144del, R190S, D215G, LA242-243del, Y449H, E484K, N501Y, D614G, H655Y, N679K, T716I, T859N	His & Avitag™	Order
SARS-CoV-2 Mu | B.1.621	SPN-C52Ha	T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N	His Tag	Order
	SPN-C82Ek	T95I, Y144S, Y145N, R346K, E484K, N501Y, D614G, P681H, D950N	His & Avitag™	Order
SARS-CoV-2 Alpha | B.1.1.7	SPN-C52Hp	N501Y, D614G	His Tag	Order
	SPN-C52H6	HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H	His Tag	Order
	SPN-C82E5	HV69-70del, Y144del, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H	His & Avitag™	Order
SARS-CoV-2 Beta | B.1.351	SPN-C52Hk	L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V	His Tag	Order
	SPN-C82E4	L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V	His & Avitag™	Order
	SPN-C52Hm	K417N, D614G	His Tag	Order
	SPN-C52Hq	E484K, D614G	His Tag	Order
SARS-CoV-2 Gamma | P.1	SPN-C52Hg	L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F	His Tag	Order
	SPN-C82E6	L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F	His & Avitag™	Order
	SPN-C52Hn	K417T, D614G	His Tag	Order
	SPN-C52Hq	E484K, D614G	His Tag	Order
SARS-CoV-2 Kappa | B.1.617.1	SPN-C52Hr	T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H	His Tag	Order
	SPN-C82E7	T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H	His & Avitag™	Order
SARS-CoV-2 B.1.620	SPN-C52Hh	P26S, HV69-70del, V126A, Y144del, LLA241-243del, H245Y, S477N, E484K, D614G, P681H, T1027I, D1118H	His Tag	Order
	SPN-C82Ed	P26S, HV69-70del, V126A, Y144del, LLA241-243del, H245Y, S477N, E484K, D614G, P681H, T1027I, D1118H	His & Avitag™	Order
SARS-CoV-2 D614G	SPN-C52H3	D614G	His Tag	Order
	SPN-CR2H3	D614G	His Tag	Order
	SPN-C82E3	D614G	His & Avitag™	Order
	SPN-C52Hm	K417N, D614G	His Tag	Order
	SPN-C52Hn	K417T, D614G	His Tag	Order

Assay data

The purity of SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) is more than 90% verified by SDS-PAGE under reducing (R) condition. The molecular weight was around 550-660 kDa confirmed by SEC-MALS. The particles are similar in size and appearance to SARS-CoV-2 trimers reported in published literature verified by negative stain electron micrography.

Protocol

Bioactivity Verification

Immobilized Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) with a linear range of 0.3-10 ng/mL.

Protocol

Comparison of Sample Test Data

SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) shows highest signal-to-noise ratio as compared to other spike proteins constructs in antibody tests in convalescent serum

Protocol

Highly efficient tools for antigen detection:RBD/S trimer antigen quantitative detection kit (sandwich ELISA)

Meet different detection needs at customized sensitivity
Antibody pairs in the kit can recognize mutant antigens from multiple strains at equal sensitivity
High batch-to-batch stability ensured consistency of detection in the mass manufacturing and quality control process

Product List

Cat.No.	Product description	sample	Preorder/Order
RAS-A010	SARS-CoV-2 Nucleocapsid Protein Titer Assay Kit	Vaccine samples	Order
RAS-A020	SARS-CoV-2 Spike Protein Titer Assay Kit	Vaccine samples	Order
RAS-A021	SARS-CoV-2 Spike RBD Titer Assay Kit	Vaccine samples	Order
RAS-A039	SARS-CoV-2 Spike Protein ELISA Kit (For Vaccine Development)	Vaccine samples	Order
RAS-A050	SARS-CoV-2 Spike RBD ELISA Kit (For Vaccine Development)	Vaccine samples	Order
RAS-A071	SARS-CoV-2 Spike Trimer (B.1.1.529) ELISA Kit (For Vaccine Development)	Vaccine samples	Order
RAS-A072	SARS-CoV-2 Spike RBD (B.1.1.529) ELISA Kit (For Vaccine Development)	Vaccine samples	Order

Assay data

>>> The activity of antibodies were verified by pseudovirus. Click to learn more.

Antigen Bioactivity Characterization

• ACE2 proteins

• Antigen-ACE2 interaction service

High quality dimeric receptor:ACE2 proteins

Available in multiple tags: His, His & Avitag™, Fc, Fc & Avitag™ etc.
Available in multiple species: Human, Mouse, Cynomolgus, Rat, Paguma larvata
Available in biotinylated or unconjugated forms that suits different experimental needs
High purity and binding activity with spike proteins verified with ELISA/SPR/BLI

Product List

Molecule	Cat.No.	Species	Tag	Host	Product description	Preorder/Order
ACE2	AC2-H82E6	Human	His Tag & Avi Tag	HEK293	Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified)	Order
	AC2-H5257	Human	Fc Tag	HEK293	Human ACE2 / ACEH Protein, Fc Tag (MALS verified)	Order
	AC2-H82F9	Human	Fc Tag & Avi Tag	HEK293	Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™	Order
	AC2-H52H8	Human	His Tag	HEK293	Human ACE2 / ACEH Protein, His Tag (MALS verified)	Order
	AC2-H5205	Human	Mouse IgG1 Fc Tag	HEK293	Human ACE2 / ACEH Protein, Mouse IgG1 Fc Tag (MALS verified)	Order
	AC2-H82E8	Human	His Tag & Avi Tag	HEK293	Biotinylated Human ACE2 / ACEH Protein, His,Avitag™	Order
	AC2-P5248	Paguma larvata	His Tag	HEK293	Paguma larvata ACE2 / ACEH Protein, His Tag (SPR verified)	Order
	AC2-M5248	Mouse	His Tag	HEK293	Mouse ACE2 / ACEH Protein, His Tag (MALS verified)	Order
	AC2-C52H7	Cynomolgus	His Tag	HEK293	Cynomolgus ACE2 / ACEH Protein, His Tag	Order
	AC2-R5246	Rat	His Tag	HEK293	Rat ACE2 / ACEH Protein, His Tag (MALS verified)	Order
Neuropilin-1	NR1-H5228	Human	His Tag	HEK293	Human Neuropilin-1 / NRP1 / CD304 Protein, His Tag	Order
	NR1-H5252	Human	Fc Tag	HEK293	Human Neuropilin-1 / NRP1 / CD304 Protein, Fc Tag	Order
	NR1-H82E3	Human	His Tag & Avi Tag	HEK293	Biotinylated Human Neuropilin-1 / NRP1 / CD304 Protein, His,Avitag™	Order
	NR1-M5254	Mouse	Mouse IgG2a Fc Tag	HEK293	Mouse Neuropilin-1 / NRP1 / CD304 Protein, Mouse IgG2a Fc Tag, low endotoxin	Order
ASGR1	AS1-H5253	Human	Fc Tag	HEK293	Human ASGR1/ASGPR1 Protein, Fc Tag	Order
KREMEN1	KR1-H5253	Human	Fc Tag	HEK293	Human Kremen-1 protein, Fc Tag	Order

Assay data

Bioactivity Verification

Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H3) at 2 μg/mL (100 μL/well) can bind Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) with a linear range of 2-20 ng/mL (QC tested).

Protocol

Human ACE2, Fc Tag (Cat. No. AC2-H5257) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind recombinant SARS-CoV-2 Spike vaccine with an affinity constant of 16.9 nM as determined in a SPR assay (Biacore T200).

Protocol

Aid comprehensive research on antigen-ACE2 interaction:Molecular interaction analytical services

Multi-platform analytical services (SPR, BLI, etc.)
Qualitative/quantitative analysis of the speed, affinity, and mechanism of binding between antigen proteins and host receptors
High-standard QC system with high-quality data output
Customized report generation in 1-2 days

>>> The proteins are with high purity verified by MALS and high bioactivity verified by in vitro assay and cell based assay. Click to learn more.

>>> If you have any customized inquiries or suggestions for SARS-CoV-2 (COVID-19) related product development, please click here.

Immune Persistence Research

• Total Antibody Titer Determination

• Neutralizing Antibody Titer Detection

• Broad-spectrum anti-RBD neutralizing antibody

In vivo antibody titer determination in preclinical & clinical trial:S trimer/RBD/S1 IgG Antibody Titer Detection Kit (indirect ELISA)

NAb kits are standardized with WHO International Standard Serum (G, NIBSC code 20/136) to ensure that data is comparable between studies and laboratories
Stable reagents with high batch-to-batch consistency to ensure data reliability for measuring changes in antibody titers at different times after vaccination
Provide different kits suitable for serum antibody detection in different species (rats, monkeys, humans) to meet the needs of preclinical and clinical experiments
Provide VOCs mutant-specific IgG antibody titer kits for antibody titer evaluation of vaccines against multiple viral variants

Application	Cat. No.	Antigen	Sample	Product Description	Preorder/Order
Preclinical trial	RAS-T018	Spike RBD	Mouse Serum	Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit	Order
	RAS-T023	Spike Trimer	Mouse Serum	Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)	Order
	RAS-T019	Spike RBD	Monkey Serum	Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit	Order
	RAS-T059	SARS-CoV-2    OmicronNEW B.1.1.529	Mouse Serum	Mouse Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG Titer Serologic Assay Kit (Spike RBD)	Preorder
	RAS-T060		Mouse Serum	Mouse Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG Titer Serologic Assay Kit (Spike Trimer)	Preorder
	RAS-T061		Mouse Serum	Mouse Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG Titer Serologic Assay Kit (Spike S1)	Preorder
	RAS-T062		Monkey Serum	Monkey Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG Titer Serologic Assay Kit (Spike RBD)	Preorder
	RAS-T063		Monkey Serum	Monkey Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG Titer Serologic Assay Kit (Spike Trimer)	Preorder
	RAS-T064		Monkey Serum	Monkey Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG Titer Serologic Assay Kit (Spike S1)	Preorder
	RAS-T070	SARS-CoV-2    Delta | B.1.617.2    (India)	Mouse Serum	Mouse Anti-SARS-CoV-2 (B.1.617.2) Antibody IgG Titer Serologic Assay Kit (Spike Trimer)	Order
Clinical trial	RAS-T024	Spike RBD	Human Serum	Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD)	Order
	RAS-T025	Spike Trimer	Human Serum	Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)	Order
	RAS-T001	Spike S1	Human Serum	Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike S1)	Order
	RAS-T008	Nucleocapsid Protein	Human Serum	Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Nucleocapsid Protein)	Order
	RAS-T009	Spike RBD	Human Serum	Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD)	Order
	RAS-T026	Alpha (B.1.1.7) Spike RBD	Human Serum	Anti-SARS-CoV-2 (B.1.1.7) Antibody IgG Titer Serologic Assay Kit (Spike RBD)	Order
	RAS-T027	Alpha (B.1.1.7) Spike protein	Human Serum	Anti-SARS-CoV-2 (B.1.1.7) Antibody IgG Titer Serologic Assay Kit (Spike Protein)	Order
	RAS-T029	Beta (B.1.351) Spike RBD	Human Serum	Anti-SARS-CoV-2 (B.1.351) Antibody IgG Titer Serologic Assay Kit (Spike RBD)	Order
	RAS-T030	Beta (B.1.351) Spike protein	Human Serum	Anti-SARS-CoV-2 (B.1.351) Antibody IgG Titer Serologic Assay Kit (Spike Protein)	Order
	RAS-T032	Gamma (P.1) Spike RBD	Human Serum	Anti-SARS-CoV-2 (P.1) Antibody IgG Titer Serologic Assay Kit (Spike RBD)	Order
	RAS-T033	Gamma (P.1) Spike protein	Human Serum	Anti-SARS-CoV-2 (P.1) Antibody IgG Titer Serologic Assay Kit (Spike Protein)	Order
	RAS-T057	SARS-CoV-2 OmicronNEW B.1.1.529 (South Africa)	Human Serum	Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG Titer Serologic Assay Kit (Spike RBD)	Preorder
	RAS-T058		Human Serum	Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG Titer Serologic Assay Kit (Spike Trimer))	Preorder
	RAS-T069		Human Serum	Anti-SARS-CoV-2 (B.1.1.529) Total Antibody Titer Serologic Assay Kit (Spike RBD)	Preorder

Assay data

Sample data

Post-vaccination serum samples are tested with Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD) (Cat.No.RAS-T024), which accurately and precisely measure antibody titer in serum (Accuracy≤±15%; Intra-assay precision<10%; Inter-assay precision <15%).

Protocol

WHO International Standard Serum (G, NIBSC code 20/136) Calibration Results

Protocol

Anti-SARS-CoV-2 Antibody IgG Quantitative Detection Kit (Spike Trimer) (Cat. No. RAS-T048) is developed for quantitative detection of IgG antibodies in post-vaccination serum. This kit is an important supplement to the existing vaccine evaluation methods which significantly improve the efficiency and accuracy of vaccine evaluation.

Protocol

High-throughput alternative for neutralizing antibody detection: Neutralizing Antibody Titer Detection Kit (competitive ELISA)

High correlation with WHO International Standard Serum (G, NIBSC code 20/136): ensures the data is comparable between studies and laboratories
High correlation with pseudovirus tests: can be used as a high-throughput alternative method for pseudovirus neutralization assay
Simple operation: the entire experiment can detect 90 + samples at the same time in 1.5-2.0 h
Mutation-specific (VOCs) neutralizing antibody detection kits: can identify mutant-specific neutralizing antibodies at high sensitivity

Lineage	Cat.No.	Product Description	sample	Preorder/Order
SARS-CoV-2 Wild Type	RAS-N022	Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit	Human Serum	Order
SARS-CoV-2 Alpha | B.1.1.7	RAS-N028	Anti-SARS-CoV-2 (B.1.1.7) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)	Human Serum	Order
SARS-CoV-2 Beta | B.1.351	RAS-N031	Anti-SARS-CoV-2 (B.1.351) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)	Human Serum	Order
SARS-CoV-2 Gamma | P.1	RAS-N034	Anti-SARS-CoV-2 (P.1) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)	Human Serum	Order
SARS-CoV-2 Delta | B.1.617.2	RAS-N040	Anti-SARS-CoV-2 (B.1.617.2) Neutralizing Antibody Serologic Assay Kit (Spike RBD)	Human Serum	Order
	RAS-N041	Anti-SARS-CoV-2 (B.1.617.2) Neutralizing Antibody Serologic Assay Kit (Spike Trimer)	Human Serum	Order
SARS-CoV-2 OmicronNEW B.1.1.529 (South Africa)	RAS-N056	Anti-SARS-CoV-2 (B.1.1.529) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD)	Human Serum	Order

Assay data

Experimental Principle

Competitive ELISA

Clinical Test Data

Post-vaccination serum NAb test (Cat. No. RAS-N022) demonstrates high sensitivity and accuracy

Pseudovirus Neutralization

16 convalescent serum samples were tested for neutralizing antibody level with pseudovirus and ELISA kit (Cat. No. RAS-N022), respectively. The results are highly correlated (R2 =93.73)。

Clinical Test Data

56 post-vaccination (inactivated vaccines) serum samples are tested for neutralizing capacity against multiple strains (Alpha, Beta, Gamma, Delta) of SARS-CoV-2 with wild type (WT) and mutant-specific kits. Neutralization against all VOCs is lower than the WT, with Delta being the lowest.

WHO International Standard Serum (G, NIBSC code 20/136) Calibration Results

Note：y = (A - D) / [1 + (x/C)^B] + D (A =1.55562 B =1.45522 C=14.42024 D=0.05532 r^2= 1)

Qualified positive control for neutralizing antibody detection:Broad-spectrum anti-RBD neutralizing antibody

High specificity and binding activity verified by ELISA
Pseudovirus-verified broad spectrum neutralization of a variety of mutants, including all the VOCs

Product List

Cat.No.	Source	Isotype	Product description	Preorder/Order
S1N-M122	Mouse	Human IgG1	Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122)	Order
SPD-M265	Human	Human IgG1	Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1	Order
SPD-M180	Human	Human IgG1	Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Human IgG1 (AM180）	Order
SAD-S35	Human	Human IgG1	Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35)	Order
SPD-M128	Mouse	Human IgG1	Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128)	Order
SPD-M162	Mouse	Human IgM	Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgM (AM122)	Order
SPD-M121	Mouse	Human IgG1	Anti-SARS-CoV-2 Spike NTD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM121)	Order
S1N-VM226	Mouse	Human IgG1	Biotinylated Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122)	Order
SPD-M201	Cynomolgus	Cynomolgus IgG1	Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Cynomolgus IgG1 (AM122)	Order
SPD-M58P1	Mouse	Human IgA1	Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgA1 (AM122)	Order
SPD-PM226	Mouse	Human IgG1	PE-Labeled Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122)	Order
SPD-M268	Human	Human IgG1	Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Human IgG1 (AM268)	Order

Assay data

>ELISA Verification

Anti-SARS-CoV-2 Spike RBD Neutralizing antibody (Cat.No.S1N-M122)neutralizes SARS-CoV-2 Spike RBD by inhibiting RBD: ACE2 interaction. The ACE2-coated plate is incubated with the wild type (WT) RBD or B.1.1.7, B.1.351, P.1, B.1.617.1, B.1.617.2 mutant and treated with the neutralizing antibody at increasing concentration. Percent inhibition is calculated based on the OD value.

Protocol

Pseudovirus verification data

As verified by SARS-CoV-2 spike pseudovirus neutralization test, the broad-spectrum neutralizing antibody (Cat. No. S1N-M122) can effectively neutralize wild-type and Alpha (B.1.1.7) /Beta (B.1.351) /Gamma (P.1) /Delta (B.1.617.2, data not shown) viral mutants, making it suitable as a quality control antibody in neutralizing antibody screening for mutant strains, neutralizing antibody detection in convalescent or vaccinated serum, etc.

Protocol

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Toxicity Studies (antibody subtype detection)

• antibody subtype detection

IgG Antibody Subtype Detection Kit (indirect ELISA):support research in ADE and ERD

Designed for serological tests of IgG subtypes: IgG1, IgG2, IgG3, and IgG4
High specificity that ensures antibodies are correctly typed
High stability and batch-to-batch consistency
The complete set of reagents needed for an experiment is provided in one kit to ease operation
Can test 90 + samples at the same time within 2h

Cat.No.	Product Description	sample	Preorder/Order
RAS-T014	Anti-SARS-CoV-2 Antibody IgG1 Titer Serologic Assay kit	Human Serum	Order
RAS-T015	Anti-SARS-CoV-2 Antibody IgG2 Titer Serologic Assay kit	Human Serum	Order
RAS-T016	Anti-SARS-CoV-2 Antibody IgG3 Titer Serologic Assay kit	Human Serum	Order
RAS-T017	Anti-SARS-CoV-2 Antibody IgG4 Titer Serologic Assay kit	Human Serum	Order
RAS-T065	Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG1 Titer Serologic Assay Kit (Spike RBD)	Human Serum	Preorder
RAS-T066	Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG2 Titer Serologic Assay Kit (Spike RBD)	Human Serum	Preorder
RAS-T067	Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG3 Titer Serologic Assay Kit (Spike RBD)	Human Serum	Preorder
RAS-T068	Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG4 Titer Serologic Assay Kit (Spike RBD)	Human Serum	Preorder

Assay data

Cross-validation data for antibody subtype detection

ACRO's products have been cited by several COVID-19 related study

ACROBiosystems is a leading manufacturer of recombinant proteins and other critical reagents to support the development of target therapeutics, vaccines and diagnostics. ACRO’s reagents have supported lots of researchers around the globe for their COVID-19 related study and have been cited in publications on Cell, Nature, Science, etc.

• Orthogonal immunoassays for IgG antibodies to SARS-CoV-2 antigens reveal that immune response lasts beyond 4 mo post illness onset

  Authors: Sasisekharan, Varun et al.

  Journal: Proc Natl Acad Sci U S A 2021

  Cited Product: SPD-C52H3 ,NUN-C5227

  Application: ELISA

  Request for Full-text

• High affinity nanobodies block SARS-CoV-2 spike receptor binding domain interaction with human angiotensin converting enzyme

  Authors: Esparza, Thomas J et al.

  Journal: Scientific reports 2020

  Cited Product: S1N-C5255,AC2-H52H8,SPD-C52H3,SPD-C52H3,SPD-C82E9,AC2-H82E6,SPD-S52H7,SPD-S52H4

  Application: Immunization, Competitive Immunopanning, SPR & Binding Assay & Phage Display Clone Screening, BLI, Binding Assay

  Request for Full-text

• Antibody Signature Induced by SARS-CoV-2 Spike Protein Immunogens in Rabbits

  Authors: Ravichandran S, Coyle EM, Klenow L, et al.

  Journal: Sci Transl Med 2020

  Cited Product: AC2-H52H8

  Application: SPR

  Request for Full-text

• Antibody-like proteins that capture and neutralize SARS-CoV-2

  Authors: Kondo T, Iwatani Y, Matsuoka K, et al.

  Journal: Science advances 2020

  Cited Product: S1N-S52H5

  Application: ELISA

  Request for Full-text

• Binding of the SARS-CoV-2 Spike Protein to Glycans

  Authors: Wei Hao,et al

  Journal: BioRxiv (preprint) 2020

  Cited Product: S1N-C52H3

  Application: glycan microarray

  Request for Full-text

• Cross-neutralization antibodies against SARS-CoV-2 and RBD mutations from convalescent patient antibody libraries

  Authors: Yan Lou. et al

  Journal: BioRxiv (preprint) 2020

  Cited Product: S1N-C52H4,SPD-S52H4,SPD-S52H5,SPD-S52H7,SPD-S52H8

  Application: BLI,ELISA

  Request for Full-text

• Deep mutational scanning of SARS-CoV-2 receptor binding domain reveals constraints on folding and ACE2 binding

  Authors: Tyler N Starr, Allison J Greaney , Sarah K Hilton, et al.

  Journal: BioRxiv (preprint) 2020

  Cited Product: AC2-H82E6

  Application: Flow cytometry

  Request for Full-text

• Flow-Mediated Susceptibility and Molecular Response of Cerebral Endothelia to SARS-CoV-2 Infection

  Authors: Kaneko N, Satta S, Komuro Y, et al. 

  Journal: Stroke 2020

  Cited Product: S1N-C82E8

  Application: ELISA

  Request for Full-text

• HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry

  Authors: Wei C, Wan L, Yan Q, et al.

  Journal: Nature metabolism 2020

  Cited Product: SPN-C52H9,S1N-C52H3,S2N-C52H5,SPD-C52H3

  Application: SPR & FACS & Confocal microscopy

  Request for Full-text

• Human Hematopoietic Stem, Progenitor, and Immune Cells Respond Ex Vivo to SARS-CoV-2 Spike Protein

  Authors: Ropa, James et al.

  Journal: Stem cell reviews and reports 2020

  Cited Product: SPN-C52H8 (This product has been taken off the shelves. An optimized version SPN-C52H9 has been launched.)

  Application: Colony Forming Unit (CFU)

  Request for Full-text

• Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19

  Authors: Tosif S, Neeland MR, Sutton P, et al.

  Journal: Nature communications 2020

  Cited Product: S1N-S52H5,S2N-C52H5,S1N-C52H3

  Application: ELISA\FACS

  Request for Full-text

• Phase I/II study of COVID-19 RNA vaccine BNT162b1 in adults

  Authors: Mulligan, Mark J et al.

  Journal: Nature 2020

  Cited Product: SPD-C82E9

  Application: Immunogenicity assessments

  Request for Full-text

• SARS-CoV-2 exposure, symptoms and seroprevalence in healthcare workers in Sweden

  Authors: Rudberg AS, Havervall S, Månberg A, et al.

  Journal: Nat Commun 2020

  Cited Product: NUN-C5227

  Application: Luminex

  Request for Full-text

• Successful transfer of anti-SARS-CoV-2 immunity using convalescent plasma in an MM patient with hypogammaglobulinemia and COVID-19

  Authors: Luetkens T, Metcalf R, Planelles V, et al.

  Journal: Blood Adv 2020

  Cited Product: S1N-C52H3

  Application: ELISA

  Request for Full-text