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Solutions for COVID-19 vaccine safety and immunogenicity evaluation
With the efforts of the scientist around the world, up until now, there are now several COVID-19 vaccines that are in use.
In the development of a COVID-19 vaccine, safety and immunogenicity evaluation are indispensable and well-established assays are required for fast and convenient assessment of the vaccine candidates.
Based on advanced technology platform in recombinant protein production, ACROBiosystems has developed a series of critical reagents for COVID-19 vaccine development and evaluation, including ELISA kits, recombinant proteins and antibodies. These featured products are suitable for IgG/M antibody titer detection, neutralizing antibody titer detection and antigen titer detection to accelerate the vaccine development.
Fig 4. Immobilized SARS-CoV-2 S protein RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG antibody with sensitivity of 24 ng/mL
Fig 1. The purity of SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) was more than 90% verified by SDS-PAGE under reducing (R) condition. The molecular weight was around 550-660 kDa confirmed by SEC-MALS. The particles are similar in size and appearance to SARS-CoV-2 trimers reported in published literature verified by negative stain electron micrography.
Fig 2. Immobilized Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) with a linear range of 0.3-10 ng/mL
Fig 3. Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL
Pfizer employed ACROBiosystems' featured biotinylated S protein RBD in the RBD-binding IgG assay during the phase 1/2 research. The interim report showed that the biotinylated S protein RBD from ACROBiosystems (Cat.No. SPD-C82E9)was bound to streptavidin-coated Luminex microspheres.[1]
Fig 7. Inhibition of Human ACE2: SARS-CoV-2 Spike RBD (HRP) binding by Anti-SARS-CoV-2 Neutralizing Antibody measured by competitive ELISA Assay.
Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody (Cat. No. TAS022-C02) (1:2 serial dilutions, from 1.25 μg/mL to 0.0012 μg/mL) was added into Human ACE2: SARS-CoV-2 Spike RBD (HRP) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting. A representative result showed that the IC50 was calculated to be 0.06 μg/mL
Fig 5. Immobilized SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 RBD Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35) with a linear range of 0.1-3 ng/mL
Fig 8. This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike protein. The kit consists of pre-coated Anti-SARS-CoV-2 Spike Protein Antibody Microplate, SARS-CoV-2 Spike protein as Control, biotin-Anti-SARS-CoV-2 Spike protein antibody and HRP-Streptavidin. Detection is performed using HRP-Streptavidin with sensitivity of 200 pg/mL.
Fig.4 cross-validation data for antibody subtype detection
>>> related article:SARS-CoV-2 Infection: Do You Know About the Antibody-Dependent Enhancement (ADE)?
Molecule | Cat. No. | Product Description |
---|---|---|
ACE2 protein | AC2-H52H8 | Human ACE2 / ACEH Protein, His Tag (MALS verified) |
AC2-H82E6 | Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified) | |
AC2-H5257 | Human ACE2 / ACEH Protein, Fc Tag (MALS verified) | |
AC2-H82F9 | Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™ | |
S trimer protein | SPN-C52H9 | SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified) |
SPN-C82E9 | Biotinylated SARS-CoV-2 S protein, His,Avitag™, Super stable trimer (MALS verified) | |
SPN-C52H3 | SARS-CoV-2 S protein (D614G), His Tag, Super stable trimer (MALS verified) | |
SPN-C82E3 | Biotinylated SARS-CoV-2 S protein (D614G), His,Avitag™, Super stable trimer (MALS verified) | |
S protein RBD | SPD-C52H3 | SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified) |
SPD-C82E9 | Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified) | |
SPD-C5259 | SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag(MALS verified) | |
SPD-C82Aa | Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG1 Fc,Avitag™(MALS verified) | |
S1 protein | S1N-C52H4 | SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified) |
S1N-C82E8 | Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified) | |
S1N-C5256 | SARS-CoV-2 (COVID-19) S1 protein (D614G), His Tag | |
S1N-C82E3 | Biotinylated SARS-CoV-2 (COVID-19) S1 protein (D614G), His,Avitag™ | |
S1 protein NTD | S1D-C52H6 | SARS-CoV-2 (COVID-19) S1 protein NTD, His Tag |
S1 protein CTD | S1D-C52H3 | SARS-CoV-2 (COVID-19) S1 protein CTD, His Tag (MALS-verified) |
S2 protein | S2N-C52H5 | SARS-CoV-2 (COVID-19) S2 protein, His Tag |
Nucleocapsid protein | NUN-C5227 | SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag |
NUN-C82E8 | Biotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His,Avitag™ |
Category | Molecule | Cat.No. | Product Description | Preorder/Order |
---|---|---|---|---|
Neutralizing antibody titer assay kit | Spike RBD | TAS-K022 | Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit | |
Spike RBD | RAS-N028 | Anti-SARS-CoV-2 (B.1.1.7) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD) | ||
Spike RBD | RAS-N031 | Anti-SARS-CoV-2 (B.1.351) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD) | ||
Spike RBD | RAS-N034 | Anti-SARS-CoV-2 (P.1) Neutralizing Antibody Titer Serologic Assay Kit (Spike RBD) | ||
Antibody titer assay kit | Spike S1 | RAS-T001 | Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike S1) | |
Spike RBD | TAS-K002 | Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike RBD) | ||
Spike Protein | TAS-K007 | Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Spike Protein) | ||
Nucleocapsid protein | RAS-T008 | Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Nucleocapsid Protein) | ||
Antibody titer assay kit | Spike RBD | RAS-T009 | Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit (Spike RBD) | |
Spike S1 | RAS-T012 | Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay kit (Spike S1) | ||
Spike RBD | RAS-T013 | Anti-SARS-CoV-2 Antibody IgM Titer Serologic Assay kit (Spike RBD) | ||
Spike RBD | RAS-T018 | Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit | ||
Spike RBD | RAS-T019 | Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit | ||
Spike RBD | RAS-T026 | Anti-SARS-CoV-2 (B.1.1.7) Antibody IgG Titer Serologic Assay kit (Spike RBD) | ||
Spike Protein | RAS-T027 | Anti-SARS-CoV-2 (B.1.1.7) Antibody IgG Titer Serologic Assay kit (Spike Protein) | ||
Spike RBD | RAS-T029 | Anti-SARS-CoV-2 (B.1.351) Antibody IgG Titer Serologic Assay kit (Spike RBD) | ||
Spike Protein | RAS-T030 | Anti-SARS-CoV-2 (B.1.351) Antibody IgG Titer Serologic Assay kit (Spike Protein) | ||
Spike RBD | RAS-T032 | Anti-SARS-CoV-2 (P.1) Antibody IgG Titer Serologic Assay kit (Spike RBD) | ||
Spike Protein | RAS-T033 | Anti-SARS-CoV-2 (P.1) Antibody IgG Titer Serologic Assay kit (Spike Protein) | ||
Antibody isotype assay kit | Spike RBD | RAS-T014 | Anti-SARS-CoV-2 Antibody IgG1 Titer Serologic Assay kit | |
Spike RBD | RAS-T015 | Anti-SARS-CoV-2 Antibody IgG2 Titer Serologic Assay kit | ||
Spike RBD | RAS-T016 | Anti-SARS-CoV-2 Antibody IgG3 Titer Serologic Assay kit | ||
Spike RBD | RAS-T017 | Anti-SARS-CoV-2 Antibody IgG4 Titer Serologic Assay kit | ||
Antigen assay kit | Nucleocapsid protein | RAS-A010 | SARS-CoV-2 Nucleocapsid Protein Titer Assay Kit | |
Spike Protein | RAS-A020 | SARS-CoV-2 Spike Protein Titer Assay Kit | ||
Spike RBD | RAS-A021 | SARS-CoV-2 Spike RBD Titer Assay Kit | ||
Inhibitor screening kit | Spike Protein | EP-105 | SARS-CoV-2 (COVID-19) Inhibitor screening Kit | |
Antigen pre-coated plate | S1 protein | SP-12 | SARS-CoV-2 Spike S1 protein-Coated Plates, Clear, 96-Well | |
S protein RBD | RP-13 | SARS-CoV-2 Spike protein RBD-Coated Plates, Clear, 96-Well |
Molecule | Cat. No. | Product Description |
---|---|---|
S protein RBD | SPD-M180 | Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody (AM180) |
SPD-M128 | Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128) | |
S1 protein | S1N-M122 | Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) |
S1N-M130 | Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) |
Category | Cat. No. | Product Description | |
---|---|---|---|
Antibody isotype | SAD-S35 | Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1(AS35) | |
SPD-S66 | Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG2(AS35) | ||
SPD-S67 | Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG3(AS35) | ||
SPD-S68 | Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG4(AS35) | ||
HRP- secondary antibody | IG1-S71 | Mouse Anti-Human IgG1 secondary antibody, Mouse IgG1, HRP | |
IG2-S72 | Mouse Anti-Human IgG2 secondary antibody, Mouse IgG1, HRP | ||
IG3-S73 | Mouse Anti-Human IgG3 secondary antibody, Mouse IgG1, HRP | ||
IG4-S74 | Mouse Anti-Human IgG4 secondary antibody, Mouse IgG1, HRP |
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