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> High quality proteins for SARS-CoV-2 related research and development SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified) (Cat.No. SPN-C52H9)
The well-folded trimeric particles of SARS-CoV-2 S protein, His Tag, Super stable trimer (SPN-C52H9) was verified by negative stain electron micrography. The particles are similar in size and appearance to SARS-CoV-2 trimers reported in published literature.
Human ACE2 / ACEH Protein, Fc Tag (MALS verified)
Cat.No. AC2-H5257
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. SPN-C52H9) with a linear range of 0.4-6 ng/mL (QC tested).
Biotinylated Human Neuropilin-1 / NRP1 / CD304 Protein, His,Avitag™
Cat.No. NR1-H82E3
Loaded Biotinylated Human Neuropilin-1, His,Avitag (Cat. No. NR1-H82E3) on SA Biosensor, can bind SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H3) with an affinity constant of 0.663 μM as determined in BLI assay (ForteBio Octet Red96e)
Bioactivity- ELISA
FACS analysis shows that the binding of SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat. No. SPD-C5259) to Vero E6 cells surface ACE2 was inhibited by increasing concentration of Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat. No. SAD-S35). The concentration of SARS-CoV-2 S protein RBD used is 5μg/ml. The IC50 is 10.33 μg/ml (Routinely tested).
Bioactivity- ELISA
FACS analysis shows that SARS-CoV-2 S protein RBD, Mouse IgG2a Fc Tag (Cat. No. SPD-C5259) can bind to Vero E6 cells surface ACE2. The concentration of SARS-CoV-2 S protein RBD is 5 μg/ml.
Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128) (Cat.No. SPD-M128)
Serial dilutions of Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb (Cat. No. SPD-M128) were incubated with luciferase/SARS-CoV-2 spike pseudoviruses. HEK293T cells stably expressing ACE2 were added after 90 min and neutralization titers (IC50) is 0.03255 nM, which is calculated as the serum dilution at which RLU were reduced by 50% compared with RLU in virus control wells after subtraction of background RLU in cell control wells.
Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128) (Cat.No. SPD-M128)
>>> Proteins for COVID-19 Serological Test Development
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