>Meso Scale Discovery Electrochemiluminescence (MSD) Service
Meso Scale Discovery Electrochemiluminescence (MSD) uses sandwich ELISA method coupled with electrochemiluminescence (ECL) detection and plate array technology to provide highly sensitive and multiplexed detection of analytes in a complex biological matrix. MSD can be used for analyzing biomarkers especially most kinds of cytokines and chemokines, perform PK/PD analysis, and carry out ADA immunogenicity testing in a broad range of sample types such as blood, serum, and tissue, etc.
MSD uses Sandwich ELISA method coupled with Electrochemiluminescence detection for multiplexed detection
QuickPlex SQ 120
ACROBiosystems’ QuickPlex SQ120 utilizes MSD’s proprietary multi-array technology to provide significantly increased sensitivity and dynamic range over standard ELISA with the added benefit of multiplexing. Fast, yet continuous, non-destructive reads produce consistent assay data with low sample volume requirements and minimal reagent consumption.
Standard Curve (Mouse Proinflammatory 10 Assays)
Fig.1 Mouse proinflammatory standard curve
Standard Curve (Human Panel 4 Assays)
Fig.2 Human proinflommatory standard curve
Fig.3 Anti-Rituximab antibodies bridging MSD for anti-drug antibody (ADA) assay development. Added the mix solution (biotinylated Rituximab at 5 µg/mL, SULFO-Rituximab at 5 µg/mL and increasing concentrations of Ant-RituximabAntibodies (Cat.NO.RIB-Y36, 100% human serum). Detection wasperformed using MSD Assay with a sensitivity of 0.97 ng/mL.
Fig.4 U-PLEX custom human biomarkar group 1 standard curve
Multiplexed and high throughput
Large dynamic range (1K to 10K fold)
High sensitivity, flexibility and precision
Small sample volume (as little as 25 uL) and low concentrations (pg/mL)
Reduced matrix effect
No selectivity towards specific antibody subclasses or species
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