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Your Position: Home > Meso Scale Discovery Electrochemiluminescence (MSD) Service

Meso Scale Discovery Electrochemiluminescence (MSD) Service

Background

Meso Scale Discovery Electrochemiluminescence (MSD) uses sandwich ELISA method coupled with electro­chemiluminescence (ECL) detection and plate array technology to provide highly sensitive and multiplexed detection of analytes in a complex biological matrix. MSD can be used for analyzing biomarkers especially most kinds of cytokines and chemokines, perform PK/PD analysis, and carry out ADA immunogenicity testing in a broad range of sample types such as blood, serum, and tissue, etc.

MSD uses Sandwich ELISA method coupled with Electrochemiluminescence detection for multiplexed detection

Platform

QuickPlex SQ 120

ACROBiosystems’ QuickPlex SQ120 utilizes MSD’s propri­etary multi-array technology to provide significantly increased sensitivity and dynamic range over standard ELISA with the added benefit of multiplexing. Fast, yet continuous, non-destructive reads produce consistent assay data with low sample volume requirements and minimal reagent consumption.

Scope

Case study
Cytokines Analysis
Analysis of proinflammatory cytokines in mouse serum

Standard Curve (Mouse Proinflammatory 10 Assays)

Fig.1 Mouse proinflammatory standard curve

Standard Curve (Human Panel 4 Assays)

Fig.2 Human proinflommatory standard curve

Drug Immunogenicity
Identifying ADA using bridging MSD assay format

Fig.3 Anti-Rituximab antibodies bridging MSD for anti-drug antibody (ADA) assay development. Added the mix solution (biotinylated Rituximab at 5 µg/mL, SULFO-Rituximab at 5 µg/mL and increasing concentrations of Ant-RituximabAntibodies (Cat.NO.RIB-Y36, 100% human serum). Detection wasperformed using MSD Assay with a sensitivity of 0.97 ng/mL.

Biomarker Profiling
Measuring inflammatory cytokines and chemokines


Fig.4 U-PLEX custom human biomarkar group 1 standard curve

Advantage

Multiplexed and high throughput

Large dynamic range (1K to 10K fold)

High sensitivity, flexibility and precision

Small sample volume (as little as 25 uL) and low concentrations (pg/mL)

Reduced matrix effect

Customizable panels/kits

Highly economical

No selectivity towards specific antibody subclasses or species

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