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Comparable to LAL method - Endpoint fluorescent assay, comparable to other chromogenic quantitative LAL methods.
High specificity - Unlike LAL Assay, as Factor G is absent from the rFC test kit, false-positive results due to β-glucan activation are not expected to occur.
Accuracy - Traceability of endotoxin standards in the kit against USP Standard (Catalog No: 1235503).
Fast time to results - 1 hours.
High sensitivity - Sensitivity range from 0.005-5 EU/mL.
Extensive validation – Verification on multiplex biological products, kinds of microplate readers and various buffer systems, comprehensive validation of specificity, sensitivity, precision, accuracy, applicability, and other aspects according to the parameters listed in the EUROPEAN PHARMACOPOEIA 11.0 and USP chapter<<1225>>.
Sustainable resource - Get rid of dependence on animal derived reagents, reduce dependence on horseshoe crab resources and fishing pressure and realize long-term supply.
Good inter batch consistency - Batch consistency of products is guaranteed due to the use of genetic recombination technology for production.
| Assay Type | FRET |
| Analyte | Endotoxin |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | 0.005 EU/mL |
| Standard Curve Range | 0.005 EU/mL-5 EU/mL |
| Assay Time | 1 hr |
| Suitable Sample Type | For the quantitative determination of endotoxin from pharmaceutical products, biologicals for injection and some media for tissue cultures. |
| Sample volume | 100 μL |
| ID | Components | Size |
| RES056-C01 | Bacterial Endotoxin Standard | 1 vial |
| RES056-C02 | Recombinant Factor C Protein | 96 tests |
| RES056-C03 | Fluorogenic Substrate | 96 tests |
| RES056-C04 | Water for Bacterial Endotoxins Test | 50 mL |
Production process (Process control)
Raw materials
Water testing
Intermediate product testing
Final product (Release testing)
Parenteral drugs & Biological products
Infusion, injection or tranfusion cells
Cell culture media
Medical devices
It is for research use only.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
Take the logarithm of the concentration of the Endotoxin working standard solution as the abscissa, take the ΔRFU as the ordinate. Fitting the standard curve with linearly model, and the correlation coefficient R should be ≥ 0.98 (QC tested).
The rFC method was employed to detect endotoxin residues in β-glucan at concentrations of 10ug/mL and 1ug/mL. No non-specific signals were detected. In contrast, the dynamic chromogenic method used for β-glucan detection resulted in the detection of endotoxin and non-specific signals. This indicates that recombinant factor C does not react with β-glucan, demonstrating the good specificity of the rFC method.
Explore our catalog of therapeutic antibody solutions to find the right products for you! We are dedicated to delivering solutions designed to help you drive innovation and push the boundaries of what therapeutic antibodies can be.
We offer a wide range of cell and gene therapy solutions starting from discovery to the clinic. Explore our wide range of proteins, antibodies, kits, and other assays to accelerate the development of your cell and gene therapy.
Organoid Toolbox is a collection of organoid solutions including ready-to-use organoids, organoid differentiation kits, and a variety of services to accelerate the progress of your drug development project.
ACROBiosystems developed a series of GMP grade cytokines under the GMP grade quality management system. Those products are all suitable for T/NK cell generation, activation, and proliferation in cell therapy research.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
A series of immune checkpoints including classic co-inhibitory and co-stimulatory receptors. The comprehensive catalog contains 100+ targets with various species and tags, and the high-quality proteins are in good batch-to-batch consistency.
To meet the needs of ADCs development, ACROBiosystems can provide: A variety of high-quality target proteins; MMPs/Cathepsin/uPA for cleavable linker; Anti-payload antibodies & anti-idiotypic antibodies for immunogenicity and PK analysis; SPR/BLI analytical and ADA development service.
Comprehensive collection of Fc receptor proteins, including their common variants, which can help expedite your antibody development.
Comprehensive cytokine targets including interleukins, growth factors, chemokines, TNFs, etc. are expressed by HEK293 to ensure their natural structure. Their high purity is verified by SDS-PAGE/HPLC/SEC-MALS and high bioactivity is verified by ELISA/SPR/BLI.
Aneuro provides innovative solutions for neuroscience research. Recombinant proteins, neural factors, pre-formed fibrils, electrophysiological electrodes, as well as Organoid Toolbox all in Aneuro aiming to advance neuroscience research, develop therapeutic interventions, and improve diagnostic methods for neurological diseases.
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