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| Assay Type | Sandwich-ELISA |
| Analyte | Capsid protein p24 |
| Format | 96T |
| Reactivity | HIV (HV1-HXB2) |
| Regulatory Status | RUO |
| Sensitivity | <7.81pg/mL |
| Standard Curve Range | 250-7.81pg/mL |
| Assay Time | 3hr 20 min |
| Suitable Sample Type | For the quantitative determination of HIV-1 p24 in Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 100 uL |
| ID | Components | Size |
| RES050-C01 | Pre-coated Anti-HIV-1 p24 Antibody Microplate | 1 plate |
| RES050-C02 | HIV-1 p24 Standard | 20 μg |
| RES050-C03 | Biotin-Anti-HIV-1 p24 Antibody | 20 μg |
| RES050-C04 | Streptavidin-HRP | 50 μL |
| RES050-C05 | 10xWashing Buffer | 50 mL |
| RES050-C06 | 2xDilution Buffer | 50 mL |
| RES050-C07 | Substrate Solution | 12 mL |
| RES050-C08 | Stop Solution | 7 mL |
| RES050-C09 | 1xLysis Buffer | 12 mL |
This kit is developed for quantitative detection of Human Immunodeficiency Virus-1 p24 (HIV-1 p24) in samples from CAR-T product preparation processing where it is used as a lentivirus vector.
It is for research use only.
Find the expiration date on the outside packaging and do not use reagents past their expiration date.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 6 simple steps:
a) Bring all reagents to room temperature(20℃-25℃) before use.
b) Add your sample to the plate and take the HIV-1 p24 as standard. The samples and standard are diluted by Dilution Buffer.
c) Wash the plate and add the Biotin-Anti-HIV-1 p24 Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add the Streptavidin-HRP diluted by Dilution Buffer to the plate.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated by the absorbance at 450 nm minus the absorbance at 630 nm to remove background disturbance before statistical analysis. The OD Value reflects the amount of bound protein.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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