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Your Position: Home > Antigens & Antibodies for Diagnostic Kit Development

Antigens & Antibodies for Diagnostic Kit Development
  
     Committed to high sensitivity & specificity

 Antigens & Antibodies for Diagnostic kit development – Dedicated to high sensitivity & specificity
Background

The diagnosis of SARS-CoV-2 infection is central to the management of viral transmission. To affirm infection, precise diagnostic tests that detect viral nucleic acids, viral antigens or serological antibodies are required. Compared with nucleic acid tests, antigen or serological antibody tests are set up for rapid screening and instant detection for their convenience to perform and the short time required for generating test results.

Rapid and accurate viral detection is the starting point to contain the COVID-19 pandemic.

Antigen tests that detect the presence of virus in nasopharyngeal fluids are used to prevent infectious spread between persons and communities. The challenge of diagnosis has upgraded due to the increasing number of cases of asymptomatic infection, which may aid inadvertent spread of the virus to others. Therefore, high test sensitivity and specificity are required to minimize the chance of false-negative test results.

Inducing immunity against SARS-CoV-2 is the next frontier for COVID-19 control.

Serological antibody tests as the complement of virus detection can indicate past infection. In these tests, IgG, IgM and neutralizing antibodies are detected qualitatively by enzyme-linked immunosorbent assay (ELISA). Serological antibody tests are helpful to assess protection against subsequent viral exposure, trace contact, and perform broad epidemiological evaluations.

The war against SARS-CoV-2 has changed because of mutants.

From D614G to Delta and Lambda, mutants have replaced the wild type and changed the war against COVID-19. Consequently, effectiveness of the existing detection methods, which were developed based on the parental type of the virus, becomes a significant question. Minimizing off-target effects becomes the key to block the spread of viral mutants.

TThe sensitivity and specificity of diagnostic kits significantly depend on the quality of the antigen or antibody reagents. To support the development of SARS-CoV-2 diagnostic tools, ACROBiosystems supply a collection of high-quality mutants-specific antigens and antibody reagents with verified high purity, bioactivity and batch-to-batch consistency.

Reagents for Antigen Test

The matched SARS-CoV-2 S/N antibody pairs are specifically designed for antigen detection kit development, which are suitable for a diverse set of applications, including ELISA, Chemiluminescence immunoassay (CLIA), Gold Immunochromatography Assay (GICA), etc. These antibody pairs are characterized with high sensitivity, specificity and consistency for antigen detection of both the wild type antigen and VOC mutants.

Product list

CategoryCat. No.SpeciesProduct Description
Nucleocapsid proteinNUN-S95HumanAnti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (AS95)Inquiry
NUN-M223MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM223)Inquiry
NUN-S46MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS46) (Trehalose free)Inquiry
NUN-S47MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47) (Trehalose free)Inquiry
S1 proteinS1N-M12A1MouseAnti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Trehalose free)HOTInquiry
S1N-M13A1MouseAnti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Trehalose free)HOTInquiry
S1N-S58MouseAnti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS58) (Trehalose free)Inquiry

These antibodies were recommended for antigen detection by double antibody sandwich.

>>>  Virus lysis buffer (Cat.No. LY-13) is available for antigen detection kit development.

>>>  If you have any bulk order inquiry, please feel free to click here.

Anti-nucleocapsid antibody pair NUN-M223/NUN-S95

Detection of multiple mutants (Delta, Lambda, etc.)

The antibody pair NUN-M223/NUN-S95 can detect multiple nucleocapsid mutants (Alpha, Beta, Gamma, Kappa, Delta, Lambda) at comparable sensitivity with the wild type antigen (<2 ng/mL) in sandwich GICA.

High sensitivity verified by inactivated virus sample and antigens

The two antibodies (NUN-M223/NUN-S95) bind at different epitopes of the SARS-CoV-2 nucleocapsid protein, significantly improving the efficiency of antigen detection. The antibody pairs can detect the inactivated virus sample at 1:38400 dilution ratio, as well as the Delta variant and wild type antigens at both 0.195 ng/mL.

The detection results of the inactivated virus sample at gradient dilution ratio

The detection results of the Delta and wild type antigens at gradient concentrations.

High specificity for SARS-CoV-2

The antibody pair NUN-M223/NUN-S95 has no cross-reactivity with the other 5 human-infecting coronaviruses (HCoV-OC43, HCoV-HKU1, HCoV-229E, HCoV-NL63, MERS) except SARS, which has over 90% homologous genes with the SARS-CoV-2 nucleocapsid protein.

Reagents for IgG/IgM Antibody Titer Test

ACROBiosystems provide Spike and Nucleocapsid antigens and antibodies specifically designed for IgG/IgM antibody titer test development and compatible with multiple platforms, i.e. ELISA, Chemiluminescence immunoassay (CLIA), Gold Immunochromatography Assay (GICA). The products below are available in bulk order or customized size.

Product list

  • Antigens for diagnostics

  • Positive control antibodies

MoleculeCat. No.HostTagRecommended applicationRecommended method
S proteinSPN-C52H7HEK293His TagDetect IgG/IgM by capture methodELISA
GICA
CLIA
Inquiry
S1 proteinS1N-C52H2HEK293His TagDetect IgG/IgM by capture methodInquiry
S1N-C82E9HEK293His,Avi tag™Detect IgG/IgM by capture methodInquiry
S2 proteinS2N-C52H2HEK293His TagDetect IgG/IgM by capture methodInquiry
RBD proteinSPD-C52H1HEK293His TagDetect total antibodies by double antigen sandwich method (detection)Inquiry
SPD-C52H2HEK293His TagDetect IgG/IgM by capture methodInquiry
SPD-C5251HEK293Mouse Fc TagDetect total antibodies by double antigen sandwich method (capture)Inquiry
SPD-C82E8HEK293His,Avi tag™Detect IgG/IgM by capture methodInquiry
Nucleocapsid proteinNUN-C5221HEK293His TagDetect IgG/IgM by capture methodInquiry
NUN-C51H2E.coliHis TagDetect IgG/IgM by capture methodInquiry
Cat. No.SpeciesProduct DescriptionSubtypeMolecule
S1N-M122MouseAnti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb (AM122)Human IgG1S protein RBDInquiry
SPD-M180HumanAnti-SARS-CoV-2 RBD Potent Neutralizing Antibody (AM180)Human IgG1S protein RBDInquiry
SAD-S35HumanAnti-SARS-CoV-2 Spike RBD Neutralizing Antibody (AS35)Human IgG1S protein RBDInquiry
SPD-M128MouseAnti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb (AM128)Human IgG1S protein RBDInquiry
S1N-M130MouseAnti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb (AM130)Human IgG1S protein RBDInquiry
SPD-M121MouseAnti-SARS-CoV-2 Spike NTD Neutralizing Antibody, Chimeric mAb (AM121)Human IgG1S1 protein NTDInquiry
SPD-M162MouseAnti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb (AM122)Human IgMS protein RBDInquiry
S2N-S86HumanAnti-SARS-CoV-2 Spike S2 Antibody (AS86)Human IgG4S2 proteinInquiry

>>>  If you have any bulk order inquiry, please feel free to click here.

>>>  If you have any custom inquiries or suggestions for SARS-CoV-2 (COVID-19) related products, please click here.

Assay data

High sensitivity

Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag (Cat. No. NUN-C5221) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.02-0.3 ng/mL (QC tested).

Broad-spectrum neutralization

Anti-SARS-CoV-2 Spike RBD Neutralizing antibody (Cat.No. S1N-M122) neutralizes SARS-CoV-2 Spike RBD by inhibiting RBD: ACE2 interaction. The ACE2-coated plate is incubated with the wild type (WT) RBD or B.1.1.7 | Alpha, B.1.351 | Beta, P.1 | Gamma, B.1.617.1 | Kappa, B.1.617.2 | Delta mutant and treated with the neutralizing antibody at increasing concentration. Percent inhibition is calculated based on the OD value.

High batch-to-batch consistency

Anti-SARS-CoV-2 Spike RBD Neutralizing antibody (Cat.No. S1N-M122) has high batch-to-batch consistency (%RSD<5%)

>>> All SARS-CoV-2 related products

>>> SARS-CoV-2 related kits

>>> Reagent for vaccine development and evaluation

>>> Any custom requests, suggestions or feedback are welcome.Click here.

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