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Request a FREE Sample of our FcRn Binding Kit!
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Limited Edition Golden Llama is here! Check out how you can get one. Limited Edition Golden Llama is here! Check out how you can get one.
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This kit is developed for determining the percentage of human mature lymphocyte subtypes, T cells, B cells, NK cells, T helper/inducer and T suppressor/cytotoxic cell subsets in human whole blood or/and cryopreserved PBMCs by flow cytometry.
It is for research use only.
Flow cytometry has become the technology of choice for the monitoring of immune cells as it provides the possibility to analyze a large number of parameters simultaneously, in a short time and for a reasonable cost.
| Assay Type | FACS |
| Analyte | mature lymphocyte subtypes, T cells, B cells, NK cells, T helper/inducer cell subsets, T suppressor/cytotoxic cell subsets |
| Format | 50T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Recommended events | 2×10⁴-1×10⁶ cells |
| Assay Time | 15min |
| Suitable Sample Type | Human PBMC |
| Marker | Subtype | Fluorochrome |
| CD3 | IgG2a | FITC |
| CD4 | IgG1 | PE-CY7 |
| CD8 | IgG1 | APC-CY7 |
| CD16 | IgG1 | PE |
| CD19 | IgG2a | APC |
| CD45 | IgG2a | Percp-Cy5.5 |
| CD56 | IgG2b | PE |
Opened kit and reconstituted reagent should be stored at 2-8 °C and the shelf life is 30 days from the date of opening.
TBNK cells gating logical and PBMC cell analysis: Set the CD45/SSC scatter plot to identify the CD45+ lymphocyte cell population (A). CD45+ as lymphocyte cell population, select the CD45+ gate, set the CD3/SSC scatter plot to identify the CD3+ T cell population (B). The CD3/CD4 scatter diagram the Th gate to identify the CD3+CD4+ helper T cell population (C). The CD3/CD8 scatter diagram Tc gate to identify the CD3+CD8+ toxic T cell population (D). The CD3/CD19 scatter diagram B gate to identify the CD3-CD19+ B cell population (E). At last the CD3/CD16+CD56 scatter plot was set to acess the CD3-CD56+CD16+ NK cell population (F).
Explore our catalog of therapeutic antibody solutions to find the right products for you! We are dedicated to delivering solutions designed to help you drive innovation and push the boundaries of what therapeutic antibodies can be.
We offer a wide range of cell and gene therapy solutions starting from discovery to the clinic. Explore our wide range of proteins, antibodies, kits, and other assays to accelerate the development of your cell and gene therapy.
Organoid Toolbox is a collection of organoid solutions including ready-to-use organoids, organoid differentiation kits, and a variety of services to accelerate the progress of your drug development project.
ACROBiosystems developed a series of GMP grade cytokines under the GMP grade quality management system. Those products are all suitable for T/NK cell generation, activation, and proliferation in cell therapy research.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
A series of immune checkpoints including classic co-inhibitory and co-stimulatory receptors. The comprehensive catalog contains 100+ targets with various species and tags, and the high-quality proteins are in good batch-to-batch consistency.
To meet the needs of ADCs development, ACROBiosystems can provide: A variety of high-quality target proteins; MMPs/Cathepsin/uPA for cleavable linker; Anti-payload antibodies & anti-idiotypic antibodies for immunogenicity and PK analysis; SPR/BLI analytical and ADA development service.
Comprehensive collection of Fc receptor proteins, including their common variants, which can help expedite your antibody development.
Comprehensive cytokine targets including interleukins, growth factors, chemokines, TNFs, etc. are expressed by HEK293 to ensure their natural structure. Their high purity is verified by SDS-PAGE/HPLC/SEC-MALS and high bioactivity is verified by ELISA/SPR/BLI.
Aneuro provides innovative solutions for neuroscience research. Recombinant proteins, neural factors, pre-formed fibrils, electrophysiological electrodes, as well as Organoid Toolbox all in Aneuro aiming to advance neuroscience research, develop therapeutic interventions, and improve diagnostic methods for neurological diseases.
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