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ActiveMax® Cytokines

ActiveMax® Cytokines
Introduction
ActiveMax® cytokines are a series of highly active recombinant cytokines developed by ACROBiosystems based on the HEK293 cells expression technology platform for immune cells, stem cells, and other cell cultures. ActiveMax® cytokines includes Activin A, CCL19, Erythropoietin, Flt-3 Ligand, G-CSF, GM-CSF, IFN-gamma, IL-4, IL-6, IL-7, LIF, Oncostatin M, SCF, TGF-beta 1, TNF-alpha, VEGF110, VEGF120, VEGF121, VEGF164, VEGF165.
Key Features

Tag free

Carrier free

High purity≥95%

High bioactivity verified by cell-based assay

Xenologous animal components free

Native conformation and modification

Low endotoxin≤1.0 EU/μg

High batch-to-batch consistency

Product List
MoleculeCat. No.SpeciesProduct DescriptionPreorder/Order
Verification Data

High purity

purity_ACA-H421b

ActiveMax® Human Activin A, Tag Free, low endotoxin (Cat. No. ACA-H421b) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

purity_GCF-H5214

ActiveMax® Human G-CSF, Tag Free (Cat. No. GCF-H5214) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

purity_TG1-H4212

ActiveMax® Human TGFB1, Tag Free (Cat. No. TG1-H4212) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

purity_VE5-H4210
purity_VE5-H4210-M1

ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%. The purity is more than 95% in HP-SEC, and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.

High bioactivity

GMF-H4214-C1

The bioactivity of ActiveMax® Human GM-CSF, Tag Free (Cat. No. GMF-H4214) was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50 < 0.1 ng/mL, corresponding to a specific activity of > 1x107 Unit/mg.

IFG-H4211-C1

The bioactivity of ActiveMax® Human IFN-gamma, Tag Free (Cat. No. IFG-H4211) was determined by dose-dependent inhibition of the proliferation of HT-29 cells. The EC50 for this effect is typically 0.41-0.74 ng/mL.

VE5-H4210-C1

Immobilized ActiveMax® Human VEGF165, Tag Free (MALS verified) (Cat. No. VE5-H4210) at 2 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Bevacizumab and 0.5 μg/mL (100 μL/well) Biotinylated Human VEGF R2, Avitag,His Tag (Cat. No. KDR-H82E5) with a half maximal inhibitory concentration (IC50) of 0.70 μg/mL.

TNA-H4211-C1

ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) induces cytotoxicity effect on the WEH1-13VAR cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is 0.007-0.014 ng/mL.

High batch-to-batch consistency

LIF-H521b

Bioactivity of three different lots of ActiveMax® Recombinant Human LIF (Cat. No. LIF-H521b) is verified by ELISA, and the result shows very high batch-to-batch consistency.

VEGF165-VE5-H4210

Bioactivity of three different lots of ActiveMax® Recombinant Human VEGF165 (Cat. No. VE5-H4210) is verified by cell-based assay, and the result shows very high batch-to-batch consistency.

TGF-Beta-TG1-H4212

Bioactivity of three different lots of ActiveMax® Recombinant Human TGF-Beta 1 TGFB1 (Cat. No. TG1-H4212) is verified by cell-based assay, and the result shows very high batch-to-batch consistency.

TNF-alpha-TNA-H4211

Bioactivity of three different lots of ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (active trimer) (MALS verified) (Cat. No. TNA-H4211) is verified by FACS, and the result shows very high batch-to-batch consistency, and there is no significant difference in activity compared with that of company R.

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