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High Quality Laminin Protein Supports Clinical iPSC Banking Pre-coating and Suspension Methods
Induced pluripotent stem cells (iPSCs) are becoming increasingly vital in developing relevant cell models and advancing regenerative medicine. Despite their potential, the considerable time and expense required for iPSC generation present ongoing challenges for researchers. The rise of iPSC banks offers a promising solution by improving access to these valuable cells and standardizing their quality and reliability. However, establishing effective iPSC banks demands overcoming critical challenges, including achieving rapid cell expansion, maintaining stemness and differentiation potential, and ensuring genomic and karyotypic stability. High-quality iPSC banks are essential for addressing these challenges and driving further research progress. In this context, our recombinant Laminin proteins offer an superior solution for iPSC banking.
RCB: Research Cell Bank PCB: Primary Cell Bank
WCB: Working Cell Bank MCB: Master Cell Bank
At the forefront of iPSC expansion and culture research, we offer innovative solutions for your iPSC banking needs. Our high-quality products, Laminin 521 and Laminin 511, are versatile and adaptable to a wide range of culture requirements. Laminin 521 features advanced pre-coating technology, while Laminin 511 offers a convenient Suspension Methods approach. Both options are engineered to support efficient large-scale iPSC cultivation, ensuring optimal results for your research needs. Whether you're focused on basic science or clinical applications, we have the ideal Laminin protein to match your specific needs and elevate your iPSC research to new heights.
Pre-coating plates with Laminin 521 creates an ideal environment for low-density single-cell seeding, enabling cells to exhibit high motility and achieve excellent clonal survival. This process promotes the formation of a tightly arranged monolayer, ensuring efficient long-term culture of human pluripotent stem cells. Laminin 521 offers a significant advantage over feeder cell-dependent methods by delivering consistent batch-to-batch performance and providing a well-defined chemical composition. By eliminating the risk of external contamination, and enhancing overall culture efficiency, Laminin 521 empowers researchers to conduct more reliable and reproducible stem cell studies.
Our Laminin 511 features an innovative Suspension Methods, eliminating the need for plate coating prior to cell seeding. This approach addresses common concerns about lengthy curing times or plates drying out, while significantly reducing material usage. By requiring only half the typical pre-coating material, Laminin 511 enables more cost-effective and resource-efficient research. Its compatibility with automated systems simplifies workflows, reducing steps and enhancing overall efficiency. Laminin 511 is the ideal choice for researchers seeking to optimize their stem cell culture processes, saving time and resources without compromising quality.
Laminin 521 (GMP-LA5H24) effectively supports the expansion of hPSC.
Laminin 521 (GMP-LA5H24) maintains the stemness of iPSC across multiple passages
Normal karyotype (46, XX) was observed in hPSCs with Laminin 521(GMP-LA5H24) coating after 10 passages.
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Matrix Proteins | Catalog No. | Product | Application |
---|---|---|---|
Laminin 521 | GMP-LA5H24 | GMP Human Laminin 521 Protein | Human PSC expansion in the clinical stage |
Laminin 521 | LA5-H5261 | Human Laminin 521 Protein, premium grade | Human PSC expansion in the pre-clinical stage |
Laminin 511 | Coming soon! | GMP Human Laminin 511 Protein (EasyScale) | Human PSC expansion in the clinical stage |
Laminin 511 | LA1-H5213 | Human Laminin 511 Protein, premium grade (EasyScale) | Human PSC expansion in the pre-clinical stage |
Vitronectin | VIN-H5117 | Human Truncated Vitronectin (VTN-N) Protein, premium grade | Supports human PSC expansion in the pre-clinical stage |
Fibronectin | FIN-H5113 | Recombinant Fibronectin fragment, premium grade | Mesenchymal stem cell attachment and proliferation |
Laminin 111 | LA1-H5269 | Human Laminin 111 Protein, premium grade | Hepatic and neural differentiation |
Laminin 121 | LA1-H5261 | Human Laminin 121 Protein, premium grade | Especially hepatic and neural cells among many other cell types |
Laminin 211 | LA8-H5263 | Human Laminin 211 Protein, premium grade | Skeletal muscle, neural and cardiac cells differentiation |
Laminin 221 | LA2-H5269 | Human Laminin 211 Protein, premium grade | Cardiac cells differentiation |
Laminin 411 | LAT-H5263 | Human Laminin 211 Protein, premium grade | Endothelial and pancreatic cells differentiation |
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For pre-clinical translational researchers, we recommend our premium-grade Laminin 521, a reliable and cost-effective option for stem cell research. Compared to other commercial extracellular matrices, it offers superior performance and value. For clinical applications, the GMP-grade Laminin 521 is designed to support large-scale iPSC manufacturing, produced under rigorous GMP standards to ensure compliance with global regulatory requirements.
Recombinant Laminin 521 offers a defined cell matrix with superior lot-to-lot consistency. It supports faster stem cell growth in homogenous monolayer compared to basement membrane extracts. In addition, it is widely used for in vitro feeder-free culture of various hPSCs while maintaining high quality.
Transferring your stem cells to Laminin 521 is straightforward, requiring no special adaptation process. Whether you’re transitioning from Matrigel, Vitronectin and other Laminin protein, we recommend adapting your stem cells over at least two passages and using adding Rock inhibitor (such as Y-27632). If adaptation proves challenging, consider using a higher concentration of Laminin 521 protein (e.g. 10 μg/ml). Once your stem cells are well adapted after several passages, you can gradually reduce the working concentration and cell density.
To passage iPSCs on Laminin 521-coated surfaces, first ensure that the coating is prepare according to the manufacturers’s instructions. Dissociate the cells using enzymes, like EDTA, Accutase, TrypLE Select. Typically, the incubation time for dissociation is approximately 5 to 6 minutes, though it may vary based on the specific cell line. Laminin 521 often results in tighter cell attachment compared to other matrix proteins, so using a lower working concentration of Laminin 521 may help reduce the detachment time.
Laminin proteins play a key role in stem cell expansion and differentiation due to their diverse tissue-specific expression . For hPSC culture, Laminin 521 and Laminin 511 are ideal for the maintaining high-quality stem cells. For differentiation into hepatic and neural cell, Laminin 111 and Laminin 121 are recommended. Laminin 211 supports differentiation into skeletal muscle, neural and cardiac cells while, Laminin 411 is suitable for endothelial and pancreatic cells differentiation.
Application Note--- High quality of Laminin 521 protein supports clinical iPSC banking
Flyer--- Research Grade Human Laminin 521
Brochure---Induced Pluripotent Stem Cells Culture and Differentiation Solutions
Brochure---Making the Transition to GMP
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