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| Assay Type | Sandwich-ELISA |
| Analyte | CCL5/RANTES |
| Format | 96T |
| Reactivity | Human |
| Sensitivity | 15.63 pg/mL |
| Standard Curve Range | 15.63 pg/mL-1000 pg/mL |
| Assay Time | 1.75 hr |
| Suitable Sample Type | For the quantitative determination of human CCL5/RANTES in Cell Culture Supernatants , Plasma, Serum. |
| Sample volume | 50 uL |
| ID | Components | Size |
| CEA088-C01 | Pre-coated Anti-CCL5 Antibody Microplate | 1 plate |
| CEA088-C02 | Human CCL5 Standard | 200 μL |
| CEA088-C03 | Biotin-Anti-CCL5 Antibody Con. Solution | 100 μL |
| CEA088-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA088-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA088-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA088-C07 | 20× Washing Buffer | 50 mL |
| CEA088-C08 | 2× Sample Dilution Buffer | 15 mL×2 |
| CEA088-C09 | Substrate Solution | 12 mL |
| CEA088-C10 | Stop Solution | 6 mL |
A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.
ClinMax™ ELISA Kits are manufactured in a GMP-like facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.
The kit is developed for quantitative detection of human CCL5 in serum, plasma and cell culture supernates.
It is for research use only.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of CCL5/RANTES is less than 15.63 pg/mL.
Ten replicates of each of 4 samples containing different CCL5/RANTES concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Five samples containing different concentrations of CCL5/RANTES were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant CCL5/RANTES was spiked into 3 human serum samples, and then analyzed. The average recovery of CCL5/RANTES for serum samples is 92.5%.
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