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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 97.5 kDa. The protein migrates as 100-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human ROBO1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human ROBO1, His Tag (Cat. No. RB1-H52H3) is more than 95% and the molecular weight of this protein is around 98-122 kDa verified by SEC-MALS.
Immobilized Anti-ROBO1 Antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human ROBO1, His Tag (Cat. No. RB1-H52H3) with a linear range of 8-125 ng/mL (QC tested).
2e5 of Anti-ROBO1 CAR-293 cells were stained with 100 μL of 10 μg/mL of Human ROBO1, His Tag (Cat. No. RB1-H52H3) and negative control protein respectively, washed and then followed by PE-anti His antibody and analyzed with FACS (Routinely tested).
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