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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 68.6 kDa. The protein migrates as 79-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human / Cynomolgus / Rhesus macaque ROR1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human / Cynomolgus / Rhesus macaque ROR1, Fc Tag (Cat. No. RO1-H5250) is more than 90% and the molecular weight of this protein is around 155-185 kDa verified by SEC-MALS.
Immobilized Human / Cynomolgus / Rhesus macaque ROR1, Fc Tag (Cat. No. RO1-H5250) at 2 μg/mL (100 μL/well) can bind Anti-ROR1 Antibody, Human IgG1 with a linear range of 0.3-5 ng/mL (QC tested).
Loaded Anti-Human ROR1 MAb (Mouse IgG1) on AMC Biosensor, can bind Human / Cynomolgus / Rhesus macaque ROR1, Fc Tag (Cat. No. RO1-H5250) with an affinity constant of 0.357 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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