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Your Position: Home > Protein > NKp30 > NC3-H5259

Human NKp30 / NCR3 / CD337 Protein, Fc Tag

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  • Synonym
    NCR3,CD337,NKp30,1C7,LY117,MALS
  • Source
    Human NKp30, Fc Tag(NC3-H5259) is expressed from human 293 cells (HEK293). It contains AA Leu 19 - Gly 135 (Accession # AAH52582).
    Predicted N-terminus: Leu 19
  • Molecular Characterization
    NKp30 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 39.5 kDa. The protein migrates as 50-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
NKp30 SDS-PAGE

Human NKp30, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 NKp30 ELISA

Immobilized Human B7-H6, His Tag (Cat. No. B76-H52H8) at 10 μg/mL (100 μL/well) can bind Human NKp30, Fc Tag (Cat. No. NC3-H5259) with a linear range of 2-78 ng/mL (QC tested).

  • Background
    Natural cytotoxicity triggering receptor 3 (NCR3) is also known as Activating natural killer receptor p30, Natural killer cell p30-related protein (NK-p30), CD antigen CD337, 1C7, LY117. NCR3 /CD337 /NKp30 belongs to the natural cytotoxicity receptor (NCR) family. NCR3 /NKp30 contains one Ig-like (immunoglobulin-like) domain. NCR3 /NKp30 is selectively expressed by all resting and activated NK cells and weakly expressed in spleen. NCR3 is homodimer in the unliganted form. NCR3 interacts with CD3Z. NCR3 interacts with and is activated by binding to NCR3LG1 or BAG6. Engagement of NCR3 by BAG6 also promotes dendritic cell (DC) maturation, both through killing those DCs that did not properly acquire a mature phenotype, and inducing NK cells to release TNFA and IFNG, which promotes DC maturation.
  • Clinical and Translational Updates

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