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Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
1A4
Mouse
Human IgG1 | Human Kappa
Unconjugated
Recombinant Monoclonal
Virus
Recombinant Influenza A [A/Hong Kong/483/97 (H5N1)] HA is expressed from human 293 cells.
Specifically recognizes Influenza A [A/Hong Kong/483/97 (H5N1)] Hemagglutinin (HA) Protein.
| Application | Recommended Usage |
| Western Blot | 2-0.1 ug/mL |
| ELISA | 0.1-63 ng/mL |
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
This product No cross-reactivity in ELISA with
Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H6).
Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3).
Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) is more than 90% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.
Immobilized Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-4 ng/mL (QC tested).
Immobilized Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Immobilized Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Immobilized Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.4-13 ng/mL (Routinely tested).
Immobilized Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-3 ng/mL (Routinely tested).
Immobilized Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.1-3 ng/mL (Routinely tested).
Immobilized Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA6-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Immobilized Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Immobilized Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H7) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Immobilized Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Influenza A (A/Hong Kong/483/1997) HA (H5N1) Antibody, Human IgG1 (1A4) (Cat. No. HA1-MY2026) with a linear range of 0.2-3 ng/mL (Routinely tested).
Detection of monoclonal Anti-Influenza A HA(H5N1),Influenza A (A/Hong Kong/483/1997) antibody-1A4, Human IgG1 | Human Kappa ,HEK by Western Blot. monoclonal Anti-Influenza A HA(H5N1), Influenza A (A/Hong Kong/483/1997) antibody-1A4, Human IgG1 | Human Kappa, HEK at 0.1ug/ml dilution + Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (MALS verified), His Tag at 400ng.
Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 80 kDa 12% Bis-Tris Protein Gel.
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