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| Assay Type | Competitive-ELISA |
| Analyte | Gentamicin |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | 0.1ng/mL |
| Standard Curve Range | 0.1 ng/mL-3.2 ng/mL |
| Assay Time | 1hr 20 min |
| Suitable Sample Type | For the detection and quantitative determination of Gentamicin residues in plasmid DNA raw materials for cell and gene therap. |
| Sample volume | 50ul |
The kit is developed for the detection of Gentamicin in drug products or semi-manufactures.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| ID | Components | Size |
| RES-A079-C01 | Gentamicin Coated Plate | 1plate |
| RES-A079-C02 | Gentamicin Standard | 0.1152ug |
| RES-A079-C03 | HRP-Anti-Gentamicin Antibody | 6mL |
| RES-A079-C04 | 1×Dilution Buffer | 50mL |
| RES-A079-C05 | 20xWashing Buffer | 50mL |
| RES-A079-C06 | Substrate Solution | 12mL |
| RES-A079-C07 | Stop Solution | 7mL |
Detection of Gentamicin by competitive ELISA Assay
Immobilized Gentamicin antigen competes with the residual Gentamicin in the sample to bind the enzyme-labeled anti-Gentamicin monoclonal (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision , Intra-Assay Precision CV≤15%.
Three samples of known concentration were tested in ten separate assays to assess inter-assay precision, Inter-Assay Precision CV≤15%.
Three samples of different concentration were tested ten times to assess Accuracy , Accuracy recovery rate 75-120%.
We have conducted interference effect test about frequently-used buffers, they have excellent buffer compatibility. For specific buffers, it is recommended that you verify recovery to determine the minimum dilution ratio.
When 500 μg/mL ampicillin, tetracycline and chloramphenicol were added into the sample diluent, no cross-reactivity was observed
High, medium and low concentrations of BSA were added to MDCK( 2×106 cells/mL), HEK293( 3.5×106 cells/mL) , CHO( 2×106 cells/mL) and T-lymphocyte( 2.15×106 cells/mL) the recovery rate of BSA was used as the specific validation index.
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