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Your Position: Home > Protein > GPRC5D > GPD-H82D5

Biotinylated Human GPRC5D Full Length Protein, Flag,His,Avitag™ (Detergent)

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  • Synonym
    GPRC5D,G-protein coupled receptor family C group 5 member D
  • Source
    Biotinylated Human GPRC5D Full Length Protein, Flag,His,Avitag(GPD-H82D5) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Cys 300 (Accession # Q9NZD1-2 ).
    Predicted N-terminus: Asp
  • Molecular Characterization
    GPRC5D Structure

    This protein carries a Flag tag at the N-terminus, followed by a polyhistidine tag and an Avi tag (Avitag™) at the C-terminus. The protein has a calculated MW of 40.1 kDa.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    This product is not suitable for cell based experiments due to cytotoxicity of DDM.
    DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.
    DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
    If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow this link for product information.
Bioactivity-ELISA
 GPRC5D ELISA

Immobilized Biotinylated Human GPRC5D Full Length Protein, Flag,His,Avitag (Cat. No. GPD-H82D5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-GPRC5D Antibody, Human IgG4 with a linear range of 0.06-4 ng/mL (QC tested).

 GPRC5D ELISA

Immobilized Anti-GPRC5D Antibody, Human IgG4 at 5 μg/mL (100 μL/well) can bind Biotinylated Human GPRC5D Full Length Protein, Flag,His,Avitag (Cat. No. GPD-H82D5) with a linear range of 0.1-16 ng/mL (Routinely tested).

 GPRC5D ELISA

Immobilized Human CD3E&CD3D Heterodimer Protein (Cat. No. CDD-H52Wa) at 10 μg/mL, add Talquetamab Biosimilar and then add Biotinylated Human GPRC5D Full Length Protein, Flag,His,Avitag (Cat. No. GPD-H82D5) at 10 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (Routinely tested).

  • Background
    G-protein coupled receptor family C group 5 member D (GPRC5D) is a retinoic acid-inducible 40 kDa protein with seven transmembrane segments and a short N terminal extracellular region. Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscle and ovary.
  • Clinical and Translational Updates

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  • Number of Drugs in Clinical Trials:19 Details
  • Latest Research Phase:Approved

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