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| ID | Components | Size |
| RAS204-C01 | Pre-coated Anti-Post-Fusion glycoprotein F0 (RSV) Antibody Microplate | 1 plate |
| RAS204-C02 | Post-Fusion glycoprotein F0 (RSV) Standard | 20 μg |
| RAS204-C03 | HRP-Anti-RSV-F0 Antibody | 20 μg |
| RAS204-C04 | 10×Washing Buffer | 50 mL |
| RAS204-C05 | 2×Dilution Buffer | 50 mL |
| RAS204-C06 | Substrate Solution | 12 mL |
| RAS204-C07 | Stop Solution | 7 mL |
An RSV F protein that has both high expression levels and maintains a stable prefusion conformation would, therefore, be a promising subunit vaccine candidate against RSV.
To facilitate the RSV-related research, drug trials and vaccine development,a rapid and effective assay kit detecting the specific levels of HRSV Post-Fusion glycoprotein F0 is urgently needed to accelerate the development of RSV vaccines.
It was developed for the specific quantitative detection of HRSV Post-Fusion glycoprotein in samples.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 5 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the Post-Fusion glycoprotein F0 (RSV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add the HRP-Anti-RSV-F0 Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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