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This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 57.9 kDa. The protein migrates as 58-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Influenza A virus (Victoria/4897/2022) NP (H1N1) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Influenza A virus (Victoria/4897/2022) NP (H1N1) Protein, His Tag (Cat. No. NP1-V5243) at 1 μg/mL (100 μL/well) can bind Pan Influenza A Nucleoprotein Antibody, Mouse MAb with a linear range of 0.1-3 ng/mL (QC tested).
Pan Influenza A Nucleoprotein Antibody, Mouse MAb immobilized on CM4 Chip can bind Influenza A virus (Victoria/4897/2022) NP (H1N1) Protein, His Tag (Cat. No. NP1-V5243) with an affinity constant of 0.118 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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