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Monoclonal Anti-DENV 2(strain Thailand/16681/1984)-NS1 Antibody, Human IgG1 (7E12) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
7E12
Human IgG1 | Human Kappa
Unconjugated
Recombinant Monoclonal
Virus
This product is a specific antibody specifically reacts with NS1.
| Application | Recommended Usage |
| Western Blot | 1:100-1:10000 |
| ELISA | 4-62.5 ng/mL |
>95% as determined by SDS-PAGE.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Monoclonal Anti-DENV 2(strain Thailand/16681/1984)-NS1 Antibody, Human IgG1 (7E12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Immobilized DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (Cat. No. NS1-D52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-DENV 2(strain Thailand/16681/1984)-NS1 Antibody, Human IgG1 (7E12) (Cat. No. DE1-M756) with a linear range of 0.06-2 ng/mL (QC tested).
Monoclonal Anti-DENV 2(strain Thailand/16681/1984)-NS1 Antibody, Human IgG1 (7E12) (Cat. No. DE1-M756) captured on Protein A Chip can bind DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (Cat. No. NS1-D52H3) with an affinity constant of 0.167 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Detection of Anti-DENV 2(strain Thailand/16681/1984)-NS1-7E12,Human,Human IgG1 | Human Kappa,HEK by Western Blot. Anti-DENV 2(strain Thailand/16681/1984)-NS1-7E12,Human,Human IgG1 | Human Kappa,HEK at 1/5000 dilution + DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (MALS verified) at 200ng.
Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.
Predicted band size: 45~55 kDa 12% Bis-Tris Protein Gel.
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