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This protein carries no "tag".
The protein has a calculated MW of 13.2 kDa. The protein migrates as 12 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid, pH2.2 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human BMP-4 (improved sequence) Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Human BMP-4 (improved sequence) Protein, premium grade (Cat. No. BM4-H5118) stimulates proliferation of Human BMP (Luc) HEK293 Reporter Cell (Cat. No. CHEK-ATF188). The specific activity of Human BMP-4 (improved sequence) Protein, premium grade is > 1.9 x 10^3 U/mg (QC tested).
Human BMP-4 (improved sequence) Protein, premium grade (Cat. No. BM4-H5118) could significantly induce iPSC derived HSPC differentiation, with high expression of CD34 and CD45 (HSPC markers) by flow cytometry analysis and the increase of viable numbers of CD34+ CD45+ cells by cell counting. NC: H2O treatment (Routinely tested).
Human BMP-4 (improved sequence) Protein, premium grade (Cat. No. BM4-H5118) could significantly inhibit endoderm cell marker FOXA2 expression and promote mesoderm cell marker Brachyury expression from iPSC differentiation by FACS analysis. NC: CHIR99021; ACRO: CHIR99021+BMP4 treatment (30 ng/mL) (Routinely tested).
Human BMP-4 (improved sequence) Protein, premium grade (Cat. No. BM4-H5118) could significantly inhibit endoderm cell marker FOXA2 expression and promote mesoderm cell marker Brachyury expression from iPSC differentiation by immunofluorescence staining. NC: CHIR99021; ACRO: CHIR99021+BMP4 treatment (30 ng/mL) (Routinely tested).
Price(USD) : $318.00
Price(USD) : $415.00
Price(USD) : $3350.00
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