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Your Position: Home > Protein > DR3 > DR3-H82F3

Biotinylated Human DR3 / TNFRSF25 Protein, Fc,Avitag™ (MALS verified)

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  • Synonym
    TNFRSF25,DR3,APO3,DDR3,TNFRSF12,WSL,WSL1
  • Source
    Biotinylated Human DR3 Protein, Fc,Avitag(DR3-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Gln 199 (Accession # Q93038-1).
  • Molecular Characterization
    DR3 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 47.2 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
DR3 SDS-PAGE

Biotinylated Human DR3 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
DR3 MALS images

The purity of Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) is more than 90% and the molecular weight of this protein is around 95-120 kDa verified by SEC-MALS.

Bioactivity-ELISA
 DR3 ELISA

Immobilized Human TL1A, His Tag (Cat. No. TLA-H5243) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a linear range of 0.005-0.625 μg/mL (QC tested).

 DR3 ELISA

Immobilized Human TL1A Protein, His Tag (Cat. No. TLA-H5244) at 5 μg/mL (100 μL/well) can bind Biotinylated Human DR3 Protein, Fc,Avitag (Cat. No. DR3-H82F3) with a linear range of 0.02-0.625 μg/mL (Routinely tested).

  • Background
    Tumor necrosis factor receptor superfamily member 25 (TNFRSF25) is also known as Apo-3, Death receptor 3 (DDR3 or DR3), Apoptosis-inducing receptor AIR, Apoptosis-mediating receptor TRAMP, Lymphocyte-associated receptor of death, Apo-3, which is a member of the TNF-receptor superfamily. TNFRSF25 is a homodimer protein, which can Interact strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. TNFRSF25 is receptor for TNFSF12 / APO3L / TWEAK.
  • Clinical and Translational Updates

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