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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 24.6 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human Fc epsilon RI alpha Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human Fc epsilon RI alpha Protein, His,Avitag (Cat. No. FCA-H82E3) is more than 90% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
Immobilized Human IgE Fc, His Tag (Cat. No. IGE-H52H9) at 1 μg/mL (100 μL/well) can bind Biotinylated Human Fc epsilon RI alpha Protein, His,Avitag (Cat. No. FCA-H82E3) with a linear range of 0.02-0.6 μg/mL (QC tested).
Loaded Biotinylated Human Fc epsilon RI alpha Protein, His,Avitag (Cat. No. FCA-H82E3) on SA Biosensor, can bind Immunoglobulin E, Human Plasma with an affinity constant of 1.92 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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