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Your Position: Home > Antibody > SN38 > SN8-PLM685

HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1

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  • Source

    HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1 is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse kappa

  • Conjugate

    HRP-Conjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Chemical

  • Immunogen

    SN38-OVA.

  • Specificity

    Specifically recognizes the target-SN38.

  • Application
    ApplicationRecommended Usage
    ELISA2-125 ng/mL 
  • Purity

    >90% as determined by SDS-PAGE.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SN38 SDS-PAGE

HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 SN38 ELISA

Immobilized ADC-SN38 at 1 μg/mL (100 μL/well) can bind HRP conjugated Monoclonal Anti-SN38 Antibody, Mouse IgG1 (Cat. No. SN8-PLM685) with a linear range of 2-31.25 ng/mL (QC tested).

  • Background
    SN-38 is an antineoplastic drug. It is the active metabolite of irinotecan (an analog of camptothecin - a topoisomerase I inhibitor) but has 1000 times more activity than irinotecan itself. In vitro cytotoxicity assays show that the potency of SN-38 relative to irinotecan varies from 2- to 2000-fold. SN38 is formed via hydrolysis of irinotecan by carboxylesterases and metabolized via glucuronidation by UGT1A1. The variant of UGT1A1 in ~10% of Caucasians which leads to poor metabolism of SN-38 predicts irinotecan toxicity, as it is then less easily excreted from the body in its SN-38 glucuronide form. SN-38 and its glucuronide are lost into the bile and intestines. It can cause the symptoms of diarrhoea and myelosuppression experienced by ~25% of the patients administered irinotecan.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $598.00

Price(USD) : $3980.00

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