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Your Position: Home > Protein > Integrin alpha 8 beta 1 > IT1-H5283

Human Integrin alpha 8 beta 1 (ITGA8&ITGB1) Heterodimer Protein, Twin-Strep Tag&Tag Free

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  • Synonym
    Integrin alpha 8 beta 1,ITGA8&ITGB1
  • Source
    Human ITGA8&ITGB1 Heterodimer Protein, Twin-Strep Tag&Tag Free(IT1-H5283) is expressed from human 293 cells (HEK293). It contains AA Phe 39 – Leu 1012 & Gln 21 - Asp 728 (Accession # P53708-1 & P05556-1).
    Predicted N-terminus: Phe 39 & Gln 21
  • Molecular Characterization
    Integrin alpha 8 beta 1 Structure

    The protein has a calculated MW of 116.4 kDa & 83.7 kDa. The protein migrates as 95-110 kDa and 150-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Integrin alpha 8 beta 1 SDS-PAGE

Human ITGA8&ITGB1 Heterodimer Protein, Twin-Strep Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Integrin alpha 8 beta 1 ELISA

Immobilized Fibronectin fragment, premium grade (Cat. No. FIN-H5113) at 5 μg/mL (100 μL/well) can bind Human ITGA8&ITGB1 Heterodimer Protein, Twin-Strep Tag&Tag Free (Cat. No. IT1-H5283) with a linear range of 0.01-0.3 μg/mL (QC tested).

  • Background
    Human Integrin alpha 8 beta 1 Heterodimer Protein consists of ITGA8 and ITGB1. The integrin alpha 8 subunit, isolated by low stringency hybridization, is a novel integrin subunit that associates with beta 1. The recently identified alpha 8 integrin subunit associates with beta 1 and is predominantly expressed in smooth muscle and other contractile cells in adult tissues, and in mesenchymal and neural cells during development. In addition, Integrin alpha 8 beta 1 is a receptor for fibronectin and can promote attachment, cell spreading, and neurite outgrowth on fibronectin.
  • Clinical and Translational Updates

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Price(USD) : $450.00

Price(USD) : $3270.00

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