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This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 46.5 kDa. The protein migrates as 60-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>85% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human TIM-3 (22-200) Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85%.
The purity of Human TIM-3 (22-200) Protein, Fc Tag (Cat. No. TM3-H5258) is more than 90% and the molecular weight of this protein is around 120-140 kDa verified by SEC-MALS.
Immobilized Human TIM-3 (22-200) Protein, Fc Tag (Cat. No. TM3-H5258) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human TIM-3 Antibody, Human IgG4 with a linear range of 0.2-2 ng/mL (QC tested).
Human TIM-3 (22-200) Protein, Fc Tag (Cat. No. TM3-H5258) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human Galectin-9, His Tag (Cat. No. LG9-H5244) with an affinity constant of 57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
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