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The kit is developed for quantitative detection of natural and recombinant human ANGPTL3 in serum, plasma and cell culture supernatants.
It is for research use only.
| Analyte | ANGPTL3 |
| Assay Type | Sandwich-ELISA |
| Reactivity | Human |
| Sensitivity | 0.156 ng/mL |
| Range | 0.156 ng/mL-10 ng/mL |
| Assay Time | 2.75 hr |
| Sample Type | Cell Culture Supernatants, Plasma, Serum. |
| Sample volume | 50 uL |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of ANGPTL3 is less than 0.156 ng/mL.
Serum – ANGPTL3 concentrations were measured in 32 human serum samples (sample dilution ratio 1:100), which were collected from healthy human subjects.
High concentrations of human ANGPTL3 serum samples were diluted with 1:2, 1:4, 1:8, 1:16 and 1:32 ratios for gradient dilution to evaluate the linearity of the assay. In the serum samples, the average detection rate of ANGPTL3 was 101.7%.
Ten replicates of each of 4 samples containing different ANGPTL3 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Five samples containing different concentrations of ANGPTL3 were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant ANGPTL3 was spiked into 3 human serum samples, and then analyzed. The average recovery of ANGPTL3 for serum samples is 100.5%.
| ID | Components | Size |
| CEA089-B01 | Pre-coated Anti-ANGPTL3 Antibody Microplate | 1 plate |
| CEA089-B02 | Human ANGPTL3 Standard | 40 μg×2 |
| CEA089-B03 | Biotin-Anti-ANGPTL3 Antibody Con. Solution | 100 μL |
| CEA089-B04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA089-B05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA089-B06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA089-B07 | 20× Washing Buffer | 50 mL |
| CEA089-B08 | 2×Sample Dilution Buffer | 15 mL×2 |
| CEA089-B09 | Substrate Solution | 12 mL |
| CEA089-B10 | Stop Solution | 6 mL |
Price(USD) : $498.00
Price(USD) :
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