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Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
7D12
Human IgG1 | Human Kappa
Unconjugated
Recombinant Monoclonal
Virus
Recombinant Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (HNN-M52H3) is expressed from human 293 cells.
Specifically recognizes Mumps virus (strain Miyahara vaccine) (MuV) HN.
| Application | Recommended Usage |
| ELISA | 0.1-8 ng/mL |
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) (Cat. No. HNN-M701) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Immobilized Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) (Cat. No. HNN-M701) with a linear range of 0.1-1 ng/mL (QC tested).
Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) (Cat. No. HNN-M701) captured on Protein A Chip can bind Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) with an affinity constant of 0.474 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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