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| Assay Type | Competitive-ELISA |
| Analyte | Gentamicin |
| Format | 96T |
| Regulatory Status | RUO |
| Sensitivity | 0.25 ng/mL |
| Standard Curve Range | 0.25 ng/mL-8 ng/mL |
| Assay Time | 1 hr 20 min |
| Suitable Sample Type | For the detection and quantitative determination of Gentamicin residues in plasmid DNA raw materials for cell and gene therap. |
| Sample volume | 50 μL |
The kit is calibrated against the USP standard.
The kit is developed for the detection and quantitative determination of gentamicin residues in plasmid DNA raw materials and proteins for CGT, vaccine and other biological drugs. It is intended for research use only (RUO).
It is for research use only.
2. The reconstructed Gentamicin standard is stored at -70°C in at least 300ul per tube and cannot be frozen or thawed repeatedly.
3. Do not use reagents past their expiration date.
| ID | Components | Size |
| RES-A025-C01 | Gentamicin Coated Plate | 1plate |
| RES-A025-C02 | Gentamicin Standard | 0.1152ug |
| RES-A025-C03 | HRP-Anti-Gentamicin Antibody | 6mL |
| RES-A025-C04 | 1×Dilution Buffer | 50mL |
| RES-A025-C05 | 20xWashing Buffer | 50mL |
| RES-A025-C06 | Substrate Solution | 12mL |
| RES-A025-C07 | Stop Solution | 7mL |
Serial dilutions of gentamicin (from 8 ng/mL to 0.25 ng/mL) was added into gentamicin: anti-gentamicin binding reactions. The assay was performed according to the protocol dscribed below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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