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This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 58.9 kDa. The protein migrates as 55-60 kDa and 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse LIGHT Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Mouse LIGHT Protein, His Tag (Cat. No. LIT-M5243) is more than 85% and the molecular weight of this protein is around 60-80 kDa verified by SEC-MALS.
Immobilized Mouse LIGHT Protein, His Tag (Cat. No. LIT-M5243) at 5 μg/mL (100 μL/well) can bind Human LTBR, Fc Tag (Cat. No. LTR-H5259) with a linear range of 0.1-2 ng/mL (QC tested).
Immobilized Mouse LIGHT Protein, His Tag (Cat. No. LIT-M5243) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LTBR, His,Avitag (Cat. No. LTR-H82E9) with a linear range of 4-31 ng/mL (Routinely tested).
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