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Your Position: Home > Protein > CD83 > CD3-H5223

Human CD83 Protein, His Tag

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  • Synonym
    CD83,HB15,hCD83,BL11
  • Source
    Human CD83, His Tag (CD3-H5223) is expressed from human 293 cells (HEK293). It contains AA Thr 20 - Ala 143 (Accession # Q01151-1).
    Predicted N-terminus: Thr 20
  • Molecular Characterization
    Online(Thr 20 - Ala 143) Q01151-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 14.9 kDa. The protein migrates as 17-18 kDa, 20-22 kDa and 25-30 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD83, His Tag (Cat. No. CD3-H5223) SDS-PAGE gel

Human CD83, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    CD83 antigen is also known as B-cell activation protein and Cell surface protein HB15. CD83 is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. CD83 is expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells. CD83 may play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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