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| ID | Components | Size |
| RAS167-C01 | Pre-coated Anti-Glycoprotein G (RABV) Antibody Microplate | 1 plate |
| RAS167-C02 | Glycoprotein G (RABV) Standard | 20 μg |
| RAS167-C03 | HRP-Anti-Glycoprotein G (RABV) Antibody | 20 μg |
| RAS167-C04 | 10xWashing Buffer | 50 mL |
| RAS167-C05 | 2xDilution Buffer | 50 mL |
| RAS167-C06 | Substrate Solution | 12 mL |
| RAS167-C07 | Stop Solution | 7 mL |
A rapid and effective assay kit detecting the levels ofRABV-G is urgently needed to accelerate the development of RABV vaccines.
This kit is developed for quantitative detection of Rabies virus Glycoprotein G in samples.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 5 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the Glycoprotein G (RABV) as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add the HRP-Anti-Pre-Fusion glycoprotein F0 (RSV) Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
Detection of Glycoprotein G (RABV) by sandwich-ELISA Assay.
Immobilized Anti-Glycoprotein G (RABV) Antibody can bind Glycoprotein G (RABV). Detection was performed using HRP- Anti-Glycoprotein G (RABV) Antibody with sensitivity of < 0.3 ng/mL (QC tested). For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.
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