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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.9 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus Apolipoprotein E Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Cynomolgus Apolipoprotein E Protein, His Tag (Cat. No. APE-C52H7) is more than 85% and the molecular weight of this protein is around 165-185 kDa verified by SEC-MALS.
Cynomolgus TREM2, His Tag (Cat. No. TR2-C52H3) immobilized on CM5 Chip can bind Cynomolgus Apolipoprotein E Protein, His Tag (Cat. No. APE-C52H7) with an affinity constant of 7.78 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Cynomolgus Apolipoprotein E Protein, His Tag (Cat. No. APE-C52H7) immobilized on CM5 Chip can bind Cynomolgus TREM2, His Tag (Cat. No. TR2-C52H3) with an affinity constant of 4.54 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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