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Your Position: Home > Protein > Glycoprotein E2 (RUBV) > GL2-R52H3

Rubella virus Glycoprotein E2 (strain M33) (RUBV), His Tag (MALS verified)

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  • Source
    Rubella virus Glycoprotein E2 (strain M33) (RUBV), His Tag(GL2-R52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 301- Ala 532 (Accession # P08563).
    Predicted N-terminus: Gly 301
  • Molecular Characterization
    Glycoprotein E2 (RUBV) Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.7 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Glycoprotein E2 (RUBV) SDS-PAGE

Rubella virus Glycoprotein E2 (strain M33) (RUBV), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Glycoprotein E2 (RUBV) MALS images

The purity of Rubella virus Glycoprotein E2 (strain M33) (RUBV), His Tag (Cat. No. GL2-R52H3) is more than 85% and the molecular weight of this protein is around 53-65 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Glycoprotein E2 (RUBV) ELISA

Immobilized Rubella virus Glycoprotein E2 (strain M33) (RUBV), His Tag (Cat. No. GL2-R52H3) at 1 μg/mL (100 μL/well) can bind Antibodies Rubella Virus E2 Antibody, Mouse IgG with a linear range of 0.1-8 ng/mL (QC tested).

  • Background
    Rubella virus (RV), the etiological agent of German measles, is a small enveloped RNA virus that belongs to the togavirus family. RV virions contain two glycosylated membrane proteins, E1 and E2, that exist as a heterodimer and form the viral spike complexes on the virion surface. Formation of an E1-E2 heterodimer is required for transport of E1 out of the endoplasmic reticulum lumen to the Golgi apparatus and plasma membrane.
  • Clinical and Translational Updates

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