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Your Position: Home > Protein > HLA-DQA1*03:02 | DQB1*03:03 > HL3-H5285

Human HLA-DQA1*03:02&DQB1*03:03 Monomer Protein (Peptide free)

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  • Source
    Human HLA-DQA1*03:02&DQB1*03:03 Monomer Protein(HL3-H5285) is expressed from human 293 cells (HEK293). It contains AA Glu 24 - Glu 217 (HLA-DQA1*03:02) & Arg 33 - Lys 230 (HLA-DQB1*03:03) (Accession # AAU88001.1 (HLA-DQA1*03:02) & AAA59755.1 (HLA-DQB1*03:03)).
  • Molecular Characterization

    Human HLA-DQA1*03:02&DQB1*03:03 Monomer Protein, produced by co-expression of HLA-DQA1*03:02 and HLA-DQB1*03:03, has a calculated MW of 27.7 kDa (HLA-DQA1*03:02) and 30.2 kDa (HLA-DQB1*03:03). Subunit HLA-DQA1*03:02 is fused with a polyhistidine tag at the C-terminus and subunit HLA-DQB1*03:03 is fused with a twin strep tag at the C-terminus. The reducing (R) protein migrates as 33-42 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HLA-DQA1*03:02 | DQB1*03:03 SDS-PAGE

Human HLA-DQA1*03:02&DQB1*03:03 Monomer Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Clinical and Translational Updates

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