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HRP conjugated Anti-His Antibody (AY63), mAb is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain.
AY63
Human IgG1 | Human Kappa
HRP-Conjugated
Recombinant Monoclonal
Tag
A protein peptide with his tag.
This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.
| Application | Recommended Usage |
| Western Blot | 0.2-2 ug/mL |
| ELISA | 1:3000-1:10000 |
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind HRP conjugated Anti-His Antibody (AY63), mAb (Cat. No. HIS-PLM535) with a linear range of 0.2-13 ng/mL (QC tested).
Detection of HRP conjugated Anti-His Tag Antibody (AY63), mAb by Western Blot. HRP conjugated Anti-His Antibody (AY63), mAb at 1/10000 dilution + Human LAG-3 / CD223 Protein, His Tag at 75-250ng.
Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.
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