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A protein peptide with his tag.
Human-Mouse Chimeric Monoclonal
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Unconjugated
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS,pH 7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63) (Cat. No. HIS-M535) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Detection of Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb by Western Blot. Monoclonal Anti-His Tag Antibody, Human IgG1 (AY63), mAb at 1/20000 dilution + Human LAG-3 / CD223 Protein , His Tag at 75-250ng. Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution. Predicted band size: 60 kDa 12% Bis-Tris Protein Gel.
Immobilized Canine TNF-alpha, His Tag (Cat. No. TNA-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-His Tag Antibody, Human IgG1(AY63) (Cat. No. HIS-M535) with a linear range of 0.1-1 ng/mL (QC tested).
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