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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.9 kDa. The protein migrates as 14 kDa and 35 kDa under reducing (R) condition (SDS-PAGE).
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse Apolipoprotein E Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.
Immobilized Human LDL R, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) with a linear range of 0.02-2.5 μg/mL (Routinely tested).
Mouse Apolipoprotein E Protein, His Tag (Cat. No. APE-M52H5) immobilized on CM5 Chip can bind Mouse LDL R, His Tag (Cat. No. LDR-M52H8) with an affinity constant of 73.4 nM as determined in a SPR assay (Biacore 8K) (QC tested).
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