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Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.
12B11E5
Mouse
Mouse IgG1 | Mouse Kappa
Unconjugated
Recombinant Monoclonal
Human
Recombinant Human IL-15 is expressed from human HEK293 cells.
This product is a specific antibody specifically reacts with IL-15, Human. No cross-reactivity is detected with other human cytokines, including IL-2, IL-4, IL-6, IL-10, IL-21,GM-CSF,TNF-alpha and IFNγ.
| Application | Recommended Usage |
| ELISA | 0.2-60 ng/mL |
>90% as determined by SDS-PAGE.
Protein A purified/ Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS,pH 7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Human IL-15, His Tag (Cat. No. IL5-H52H8) at 1 μg/mL (100 μL/well) can bind Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 (Cat. No. IL5-M542) with a linear range of 0.2-4 ng/mL (QC tested).
Anti-Human IL-15 (12B11E5) mAb, Mouse IgG1 (Cat. No. IL5-M542) captured on CM5 chip via anti-mouse antibodies surface can bind Human IL-15, His Tag (Cat. No. IL5-H52H8) with an affinity constant of 0.138 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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