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| Items | Details |
| Detection Method | Chemiluminescence |
| Product Type | Magnetic Beads (Streptavidin) |
| Quantity Size | 10mg / 100mg |
| Physical Appearance | lyophilized powder mixture |
| Particle size | 2.8µm |
| Beads Surface | Hydrophilic |
| Amount of Coupled Protein | About 600 pmol Streptavidin / mg Beads |
| Binding Capacity | 5-20 μg biotinylated proteins or molecules / mg Beads |
| Emission Wavelength | Measured relative light units (RLU) at 430 nm |
See Certificate of Analysis (CoA) for detailed instruction.
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Immobilized 0.04 μg /Test of Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) to the Streptavidin-Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A006, 10 μg beads/Test), incubated with 100 μL /Test of Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) at increasing concentration coupled to Anti-Mouse IgG-Acridine ester (Cat. No. AMG-S151, 0.04 μg /Test). Detection was performed with sensitivity of 0.06 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
The MPCLIA assay shows that Streptavidin-Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A006) is stable at 37°C for 10 days.
The MPCLIA assay shows that Streptavidin-Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A006) is stable after freezing and thawing 3 times.
The MPCLIA assay shows that Streptavidin-Magnetic Beads (used for MPCLIA) (Cat. No. MPC-A006) is stable at 4°C for 30 days.
Immobilized 0.04 μg /Test of Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) to the Streptavidin-Magnetic Beads (recommended for MPCLIA) (Cat. No. MPC-A006, 20 μg beads/Test), incubated with 100 μL /Test of Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) at increasing concentration coupled to Anti Mouse IgG-Acridine ester (Cat. No. AMG-S163, 0.04 μg /Test). Detection was performed with sensitivity of 0.98 ng/mL in Magnetism particulate chemiluminescence immunoassay (MPCLIA) (KEYSMILE, SMART 6500S) (QC tested).
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