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| ID | Components | Size |
| RAS102-C01 | Pre-coated Anti-Glycoprotein E (VZV) Antibody Microplate | 1 plate |
| RAS102-C02 | Glycoprotein E (VZV) Standard | 30 μg |
| RAS102-C03 | Biotin-Anti-Glycoprotein E (VZV) Antibody | 20 μg |
| RAS102-C04 | Streptavidin-HRP | 50 μL |
| RAS102-C05 | 10xWashing Buffer | 50 mL |
| RAS102-C06 | 2xDilution Buffer | 50 mL |
| RAS102-C07 | Substrate Solution | 12 mL |
| RAS102-C08 | Stop Solution | 7 mL |
This kit is developed for quantitative detection of Varicella Zoster Virus Glycoprotein E (VZV gE) in vaccine samples.
It is for research use only.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 6 simple steps:
a) All reagents were returned to room temperature(20°C-25°C) before use.
b) Add your sample to the plate, take the Glycoprotein E (VZV) as standard sample. The samples and standard sample are diluted by Dilution Buffer.
c) Wash the plate and add a diluted Biotin-Anti-Glycoprotein E (VZV) Antibody to the plate. The diluted by Dilution Buffer.
d) Wash the plate and add a diluted Streptavidin-HRP to the plate. The diluted by Dilution Buffer.
e) Wash the plate and add TMB.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
Detection of Monoclonal Glycoprotein E (VZV) titer by sandwich-ELISA Assay.
Immobilized Anti-Glycoprotein E (VZV) Antibody can bind Glycoprotein E (VZV). Detection was performed using Biotin-Anti-Glycoprotein E (VZV) Antibody with sensitivity of 78 pg/mL (QC tested).
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