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| ID | Components | Size |
| EP135-C01 | High-bind Plate | 1 plate |
| EP135-C02 | Human CD30L | 15 μg |
| EP135-C03 | Biotinylated Human CD30 | 10 μg |
| EP135-C04 | Anti-CD30 Neutralizing Antibody | 20 μg |
| EP135-C05 | Streptavidin-HRP | 10 μg |
| EP135-C06 | Coating Buffer | 12 mL |
| EP135-C07 | 10xWashing Buffer | 50 mL |
| EP135-C08 | Blocking Buffer | 50 mL |
| EP135-C09 | Substrate Solution | 12 mL |
| EP135-C10 | Stop Solution | 7 mL |
This kit is developed for screening for inhibitors of human CD30 binding to human CD30L.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human CD30 Ligand.
b) Add your molecule of interest to the tests.
c) Add human CD30-Biotin to bind the coated CD30 Ligand.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to CD30 Ligand: CD30 binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF CD30[Biotinylated]: CD30L BINDING BY ANTI-CD30 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-CD30 Neutralizing antibody (Catalog # EP135-C04) (1:1 serial dilution, from 5 μg/mL to 0.019μg/mL) was added into Biotinylated CD30: CD30L binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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