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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 69.6 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human gp130, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human gp130, His Tag (Cat. No. ILT-H52H2) is more than 90% and the molecular weight of this protein is around 85-105 kDa verified by SEC-MALS.
Immobilized Biotinylated Human IL-6 R alpha, Fc,Avitag (Cat. No. ILR-H82F9) at 10 μg/mL (100 μL/well) on Human IL-6, premium grade (Cat. No. IL6-H4218) precoated (0.5 μg/well) plate can bind Human gp130, His Tag (Cat. No. ILT-H52H2) with a linear range of 0.005-0.313 μg/mL (QC tested).
Immobilized Human gp130, His Tag (Cat. No. ILT-H52H2) at 5 μg/mL (100 μL/well) can bind Human IL-6, premium grade (Cat. No. IL6-H4218) in the presence of Biotinylated Human IL-6 R alpha, Fc,Avitag (Cat. No. ILR-H82F9) with a linear range of 0.6-20 ng/mL (Routinely tested).
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