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Your Position: Home > Protein > IL-18 R beta > ILB-H82E8

Biotinylated Human IL-18 R beta / IL-1 R7 Protein, His,Avitag™

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  • Synonym
    IL-18 R beta,IL-1 R7,CD218b,CDw218b,IL-1 R7,IL-18 R beta,IL-18 receptor accessory protein,IL-18 receptor beta,IL18R beta,IL-18RAcP
  • Source
    Biotinylated Human IL-18 R beta, His,Avitag (ILB-H82E8) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Arg 356 (Accession # O95256-1).
    Predicted N-terminus: Phe 20 - Arg 356
  • Molecular Characterization
    Online(Phe 20 - Arg 356) O95256-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 42.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-18 R beta, His,Avitag (Cat. No. ILB-H82E8) SDS-PAGE gel

Biotinylated Human IL-18 R beta, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    IL-18 R beta (IL-1R7), also known as IL18RAP (Interleukin 18 Receptor Accessory Protein), is a member of the IL-1 family. Within the IL18 receptor complex, does not mediate IL18-binding, but involved in IL18-dependent signal transduction, leading to NF-kappa-B and JNK activation.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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