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| ID | Components | Size |
| EP129-C01 | High-bind Plate | 1 plate |
| EP129-C02 | Human IL-7 R alpha & TSLP R | 25 μg |
| EP129-C03 | Anti- TSLP Neutralizing Antibody | 20 μg |
| EP129-C04 | Human TSLP-Biotin | 10 μg |
| EP129-C05 | Streptavidin-HRP | 10 μg |
| EP129-C06 | Coating Buffer | 12 mL |
| EP129-C07 | 10xWashing Buffer | 50 mL |
| EP129-C08 | Blocking Buffer | 50 mL |
| EP129-C09 | Substrate Solution | 12 mL |
| EP129-C10 | Stop Solution | 7 mL |
This kit is developed for screening for inhibitors of TSLP binding to human IL-7Rα & TSLP R.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-7 R alpha & TSLP R.
b) Add your molecule of interest to the tests.
c) Add human TLSP-Biotin to bind the coated human IL-7 R alpha & TSLP R.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit TSLP: IL-7 R alpha & TSLP Rbinding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF TSLP [BIOTINYLATED]: IL-7 R ALPHA & TSLP R BINDING BY ANTI-TSLP NEUTRALIZING ANTIBODY
Serial dilutions of Anti-TSLP Neutralizing antibody (Catalog # EP129-C03) (1:1 serial dilution, from 8 μg/mL to 0.0156 μg/mL (55.317-0.108 nM)) was added into IL-7 R alpha & TSLP R: TSLP-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
INHIBITION OF TSLP [BIOTINYLATED]: IL-7 R ALPHA & TSLP R BINDING BY ANTI- TSLP & IL-13 BISPECIFIC ANTIBODY
Serial dilutions of anti TSLP & IL-13 bispecific antibody (1:1 serial dilution, from 8 μg/mL to 0.0156 μg/mL (55.317-0.108 nM)) was added into IL-7 R alpha & TSLP R: TSLP-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (Routinely tested).
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