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This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 16.0 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Monkeypox virus (strain Zaire-96-I-16) A30L, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Monkeypox virus (strain Zaire-96-I-16) A30L, His Tag (Cat. No. A3L-M5243) is more than 90% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.
Immobilized Monkeypox virus (strain Zaire-96-I-16) A30L, His Tag (Cat. No. A3L-M5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Monkeypox-A30L Antibody, Human IgG1 (5F3) with a linear range of 0.1-2 ng/mL (QC tested).
Monoclonal Anti-Monkeypox-A30L Antibody, Human IgG1 (5F3) captured on Protein A Chip can bind Monkeypox virus (strain Zaire-96-I-16) A30L, His Tag (Cat. No. A3L-M5243) with an affinity constant of 0.601 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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