This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.4 kDa. The protein migrates as 25-33 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD32b/c, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD32b/c, His Tag (Cat. No. CDB-H5228) was more than 90% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.
Immobilized Human CD32b/c, His Tag (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 10.1 μM as determined in a SPR assay (Biacore T200) (QC tested).
Immobilized Rituximab on CM5 Chip, can bind Human CD32b/c, His Tag (Cat. No. CDB-H5228) with an affinity constant of 5.5 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human CD32b/c, His Tag (Cat. No. CDB-H5228) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 4.30 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Rituximab on FAB2G Biosensor, can bind Human CD32b/c, His Tag (Cat. No. CDB-H5228) with an affinity constant of 5.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Simonov V, et al
Journal: Biologicals 2019
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