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Your Position: Home > Protein > GPRC5D > GPD-C82D4

Cynomolgus GPRC5D Protein, Flag,His Tag (Nanodisc)

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  • Synonym
    GPRC5D
  • Source
    Cynomolgus GPRC5D Protein, Flag,His Tag(GPD-C82D4) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Phe 192 & Glu 197 - Leu 262 (Accession # A0A2K5W6I7-1).
    Predicted N-terminus: Asp
  • Molecular Characterization
    GPRC5D Structure

    This protein carries a Flag tag at the N-terminus and a polyhistidine tag at the C-terminus. The protein has a calculated MW of 51.9 kDa. The protein migrates as 37 kDa under reducing (R) condition (SDS-PAGE). The membrane scaffold protein (MSP1D1) has calculated MW of 24.7 kDa, and it migrates as 25 kDa under reducing (R) condition (SDS-PAGE).

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by a membrane scaffold protein (MSP1D1) coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein(MSP1D1) upon removal of the detergent.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >80% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow this link for product information.
SDS-PAGE
GPRC5D SDS-PAGE

Cynomolgus GPRC5D Protein, Flag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80%.

Bioactivity-SPR
 GPRC5D SPR

Anti-GPRC5D antibody immobilized on CM5 Chip can bind Cynomolgus GPRC5D Protein, Flag,His Tag (Cat. No. GPD-C82D4) with an affinity constant of 0.134 μM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    The protein encoded by this gene is a member of the G protein-coupled receptor family; however, the specific function of this gene has not yet been determined.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:19 Details
  • Latest Research Phase:Approved

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