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Your Position: Home > Antibody > Spike RBD > SPD-M334

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (MALS verified)

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  • Source
    Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (SPD-M334) is a mouse monoclonal antibody recombinantly expressed from HEK293 cells. The antibody is specific against the Beta (B.1.351) and Gamma (P.1) variant of SARS-CoV-2, and has no binding with the spike RBD of the wild type virus, the Alpha (B.1.1.7) variant, the Delta (B.1.617.2) variant and the Omicron (B.1.1.529, BA.2) variant.
  • Clone

    AM334

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y) erived from human 293 cells.

  • Specificity

    This product is a specific antibody specifically reacts with SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y) (Cat. No.SPD-C52Hp), Gamma (Cat. No. SPD-C52Hr), B.1.1.529/Omicron (Cat. No. SPD-C522e), BA.2/Omicron (Cat. No. SPD-C522g), BA.3/Omicron (Cat. No. SPD-C522i). No cross-reactivity is detected with Spike RBD of WT (Cat. No. SPD-C52H3), Alpha (Cat. No. SPD-C52Hn), Delta (Cat. No. SPD-C52Hh), BA.4&5Omicron (Cat. No. SPD-C522r), XBB/Omicron (Cat. No. SPD-C5241) and XBB.1.5/Omicron (Cat. No. SPD-C5242).

  • Application
    ApplicationRecommended Usage
    ELISA0.06-5000 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified/ Protein G purified

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
Spike RBD SDS-PAGE

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Spike RBD MALS images

The purity of Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) is more than 90% and the molecular weight of this protein is around 130-155 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike RBD ELISA

Immobilized SARS-CoV-2 spike protein (Beta, Cat. No. SPN-C52Hk and Gamma, Cat. No. SPN-C52Hg) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) with a linear range of 0.1-8 ng/mL. The antibody does not bind spike protein of WT (Cat. No. SPN-C52H7), Alpha (Cat. No. SPN-C52H6) and Delta (Cat. No. SPN-C52He) (QC tested).

 Spike RBD ELISA

Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) can bind SARS-CoV-2 spike protein (Beta, Cat. No. SPN-C52Hk and Gamma, Cat. No. SPN-C52Hg) with a linear range of 0.5-8 ng/mL. The antibody does not bind spike protein of WT (Cat. No. SPN-C52H7), Alpha (Cat. No. SPN-C52H6) and Delta (Cat. No. SPN-C52He) (Routinely tested).

Bioactivity-BLI
 Spike RBD BLI

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor can bind SARS-CoV-2 S protein, His Tag (Beta, Cat. No. SPN-C52Hk) with an affinity constant of 11.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Spike RBD BLI

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor can bind SARS-CoV-2 S protein, His Tag (Gamma, Cat. No. SPN-C52Hg) with an affinity constant of 13.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Spike RBD BLI

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor does not bind SARS-CoV-2 S protein, His Tag (WT, Cat. No. SPN-C52H7) as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 Spike RBD BLI

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor does not bind SARS-CoV-2 S protein, His Tag (Alpha, Cat. No. SPN-C52H6) as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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