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Your Position: Home > Kits > Spike S1 > RAS-T047

Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike S1)

For research use only.

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IDComponentsSize
RAS047-C01Pre-coated SARS-CoV-2 Spike S1 Microplate1 plate
RAS047-C02Anti-SARS-CoV-2 Antibody (Control, Monkey IgG)100 μL
RAS047-C03SARS-CoV-2 Antibody Positive Control100 μL
RAS047-C04SARS-CoV-2 Antibody Negative Control100 μL
RAS047-C05HRP-Conjugated Antibody100 μL
RAS047-C0610×Washing Buffer50 mL
RAS047-C07Dilution Buffer50 mL
RAS047-C08Substrate Solution12 mL
RAS047-C09Stop Solution7 mL
  • Background
    Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide. The SARS-CoV-2 pandemic presents an extraordinary challenge to the lives and health of the global population, resulting in a total of over 200 million diagnosed cases and 4.9 million death (as of October 2021). It also significantly affected the social, economic and environmental domains throughout the world and changed everyone’s life.

    ACROBiosystems, a leading life science company dedicated to aid the global COVID-19 response,developed different kits suitable for serum antibody detection in different species (Mouse, Monkeys, humans) to meet the needs of preclinical and clinical experiments.

  • Application

    This kit is developed for qualitative, titer or semi-quantitative measurement of Anti-SARS-CoV-2 Antibody IgG (Spike S1) in Monkey serum.

    It is for research use only.

  • Storage & Shipping
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Shipping Statement

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Monkey serum by SARS-CoV-2 Spike S1 . The kit consists of Pre-coated SARS-CoV-2 Spike S1 Microplate, an Anti-SARS-CoV-2 Antibody (Control, Monkey IgG), an Positive Control, an Negative Control, an HRP-Anti-Goat anti monkey IgG secondary antibody and related buffer.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Anti-Goat anti monkey IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data Please refer to Ds document for the assay protocol.
 Spike S1 TYPICAL DATA

Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Monkey IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike S1 at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Monkey IgG in 1:50 monkey serum. Detection was performed using HRP-Goat anti-Monkey IgG antibody with sensitivity of 98 ng/mL (QC tested).

  • Clinical and Translational Updates

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Price(USD) : $580.00

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