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Your Position: Home > All Other Proteins > Klotho beta > KLB-M52H7

Mouse Klotho beta / KLB Protein, His Tag

  • Synonym
    betaKlotho,beta-klotho,BKL,KLB,klotho beta like,klotho beta-like protein
  • Source
    Mouse Klotho beta, His Tag (KLB-M52H7) is expressed from human 293 cells (HEK293). It contains AA Phe 53 - Leu 995 (Accession # Q99N32-1).
    Predicted N-terminus: Phe 53
  • Molecular Characterization
    Online(Phe 53 - Leu 995) Q99N32-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 110.8 kDa. The protein migrates as 125-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse Klotho beta, His Tag (Cat. No. KLB-M52H7) SDS-PAGE gel

Mouse Klotho beta, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human FGF19, Fc Tag (Cat. No. FG9-H5253) on Protein A Biosensor, can bind Mouse Klotho beta, His Tag (Cat. No. KLB-M52H7) with an affinity constant of 78.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human FGF R4, Fc Tag (Cat. No. FG4-H5253) on Protein A Biosensor, can bind Mouse Klotho beta, His Tag (Cat. No. KLB-M52H7) with an affinity constant of 54.0 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

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